To observe T lymphocyte subsets in peripheral blood in patients with hepatitis B and the replication of serum hepatitis B virus (HBV-DNA), to explore the relationship between the viral replication degree and cellular immune function. The relative percentage of T lymphocyte subsets in peripheral blood of 142 patients with hepatitis B and 35 healthy controls was analyzed by flow cytometry. The serum load of HBV-DNA was measured by fluorescence quantitative polymerase chain reaction (PCR). Compared with healthy controls, the percentage of CD4+ T cells in patients with chronic hepatitis B were significantly decreased and the percentage of CD8+ T cells were increased (P <0.01). Compared with healthy controls, the CD4+ /CD8+ ratio in patients with cirrhosis were decreased and the percentage of CD4 + CD25+ regulatory T cells were increased (P <0.05). Chronic hepatitis B infection can lead to dysfunction of cellular immunity; HBV-DNA high replication status can further promote the cellular immune dysfunction. The dynamic detection of CD4+/CD8+ ratio can provide the basis for clinical immunotherapy.%观察乙型肝炎病毒(HBV)感染者外周血T细胞亚群功能变化与其血清HBV-DNA水平,探讨病毒复制程度与细胞免疫功能的关系.应用流式细胞术直接荧光法检测142例HBV感染者和35名健康对照者外周血中T淋巴细胞亚群百分率,用荧光定量PCR法检测HBV感染者血清HBV-DNA.结果显示:与健康对照组比较,慢性乙型肝炎组CD4+细胞数量降低,CD8+细胞数量明显升高(P均<0.01),肝硬化组CD4+/CD8+细胞比值与健康对照组比较明显降低,CD4+ CD25+调节性T细胞数量明显增加(P<0.05).结论:慢性乙型肝炎患者可导致体内细胞免疫功能紊乱,HBV-DNA高复制状态可进一步促进细胞免疫功能紊乱,CD4+/CD8比值的动态检测可为指导临床免疫治疗提供依据.
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