Objective:To investigate the metabolic changes of central monoamine neurotransmitters in the prolonged exhaustive exercise,in order for the central mechanism of sports fatigue to provide a laboratory basis.Method:The C57/BL mice were 4 months of age,and randomly divided them into the sedentary control group(SC)and exhausted swimming group(SE).After exhaustive swimming the cortex,hypothalamus,striatum,hippocampus, brainstem and cerebellum were collected immediately.The brain monoamines levels(NE,DA,DOPAC,5-HT,5-HIAA)were measured with HPLC. Result:In exhaustive group,the concentrations of NE in the cerebral cortex,hippocampus and brainstem were significantly increased compared with the sedentary control group(P<0.05),but the concentrations of NE in the hypothalamus,striatum and cerebellum had a decreasing tendency.The levels of DA in the hippocampus and brainstem were significantly increased compared to those in the sedentary control group(P<0.01),in the cortex and striatum only had a increasing tendency,but the concentrations of DA in the hypothalamus was significantly increased compared to that in the sedentary control group.The levels of DOPAC only were increased in the brainstem.The levels of 5-HT in all six brain loci of SE group were significantly increased compared with those of SC group(P<0.05),and the metabolic product 5-HIAA were significantly increased in the cerebral cortex,hypothalamus and cerebellum (P<0.05).Conclusion:Exercise results in a different activation degree of brain monoamine neurons in brain loci.Exercise-induced increased 5-HT and NE levels can be indicators of central fatigue.%目的：探讨力竭运动中枢单胺类神经递质的代谢变化特点，以期为运动疲劳的中枢机制提供一定实验室依据。方法：4月龄C57/BL小鼠，随机分为安静对照组（SC组）和一次性游泳力竭组（SE组）。力竭游泳即刻取材（皮层、下丘脑、纹状体、海马、脑干和小脑）六个脑区，高功率微波灭活脑组织相关酶类；HPLC测定去甲肾上腺素（NE）、多巴胺（DA）及其代谢产物多巴克（DOPAC）、5羟色胺（5-HT）、5-羟吲哚乙酸（5-HIAA）含量。结果：力竭时皮层、海马和脑干NE含量较安静时明显增加（P<0.05），而下丘脑、纹状体和小脑NE含量有降低趋势。海马、脑干组织DA含量力竭时极显著增加（P<0.01），皮层、纹状体区仅有增加趋势，而下丘脑DA含量较安静时有降低趋势；DA的代谢产物DOPAC仅在脑干有明显增加。各脑区力竭时5-HT均较安静时明显增加（P<0.05），其代谢产物5-HIAA在皮层、下丘脑及小脑也增加显著（P<0.05）。结论：运动至力竭过程中各脑区单胺类神经元激活程度不相同，其中5-HT和NE可以作为中枢皮层疲劳的一个标志。
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