目的:建立全蝎的质量控制方法用于质量评价.方法:对全蝎水分、总灰分、酸不溶性灰分进行检查;考察不同浸出物溶剂对测定结果的影响;采用TLC法对全蝎中脂类成分进行定性鉴别;采用HPLC法测定全蝎中特征氨基酸的含量.结果:拟定了水分、总灰分、酸不溶性灰分限量;浸出物测定以乙醇为溶剂更为科学;TLC色谱斑点清晰;甘氨酸、精氨酸、L-脯氨酸、亮氨酸分别在0.509 1 ～5.091 0 μg(r=1.0000)、0.502 5～5.025 0 μg(r=1.000 0)、0.348 2～3.482 0 μg(r=1.000 0)、0.508 8～5.088 0μg(r=1.000 0)与峰面积呈良好的线性关系,平均回收率分别为102.5％(RSD=2.7％,n=9),104.1％ (RSD =2.8％,n=9),102.5％(RSD=2.2％,n=9),102.3％(RSD =2.9％,n=9).结论:本法简便、准确、重复性好,可用于全蝎的质量控制.%Objective:To establish the quality standard for scorpion used for quality evaluation.Methods:The contents of moisture,total ash and acid insoluble ash were determined.The effect of different extract solvents on the determination was investigated.Lipid components of scorpion were identified by TLC,the contents of characteristic amino acids were determined by HPLC.Results:The content of moisture,total ash and acid insoluble ash had been measured.The extract determination was more reasonable with ethanol as the solvent.The developed TLC spots were obvious.The method had good linear relationship within the range of 0.509 1-5.091 0 μg for glyeine(r =1.000 0),0.502 5-5.025 0 μg for arginine (r =1.000 0),0.348 2-3.482 0 μg for L-proline(r =1.000 0)and 0.508 8-5.088 0μg for leucine(r =1.000 0).The average recoveries were 102.5％,104.1％,102.5％ and 102.3％ with RSD of 2.7％,2.8％,2.2％ and 2.9％ (n =9),respectively.Conclusion:The method is simple,accurate and specific,and can be used in the quality control of scorpion.
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