以菊芋叶为原料,研究了甲醇体积分数、提取温度、提取时间、固液比等因素对菊芋叶总黄酮含量的影响,在单因素试验的基础上,以L9(34)正交试验方法优化总黄酮提取工艺,然后在该工艺条件下研究不同采收时期对菊芋叶总黄酮含量的影响.结果表明:甲醇体积分数、提取温度、提取时间、固液比对菊芋叶总黄酮提取含量均有影响,最佳工艺组合为:用体积分数70％的甲醇,固液比1:30 (g/mL),80℃条件下提取2h,在此条件下,菊芋叶总黄酮含量为7.03％.采收时间对菊芋叶总黄酮含量影响较大,9月份总黄酮含量最高.%Leaves of Helianthus tuberosus were used as the raw material to extract total flavonoids. To maximize the extraction of total flavonoids content,technological parameters such as methanol concentration,temperature,extraction time and solid/liquid ratio were investigated by single factor method coupled with L9 (34) orthogonal array design. The dynamic change of total flavonoids in leaves of H. tuberosus during growth was also examined. The results showed that the maximum extraction of total flavonoids content was obtained by using 70% methanol to extract leaves of H. tuberosus for 2 h at 80 ℃ with a solid/liquid ratio of 1: 30. Using this technology, the total flavonoids content in H. tuberosus leaves was 7.03%.The total flavonoids content showed an obvious change along with H. tuberosus growing,and the maximum content was observed in September.
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