Objective :To investigate the effect and mechanism of berberine on migration of hepatocellular car -cinoma cell line HepG2 .Method :Effcet of berberine in different concentration on HepG2 cell viability was detected by MTT .Cell migration assay was performed to demonstrate the effect of berberine in a low concentration (10μmol/L) on HepG2 cell migration and role of 3-MA in the effect of berberine on HepG2 cell migration in vitro .10μmol/L berberine inducing HepG2 cell autophagy was demonstrated by western blot .Results :Berberine in high concentrations (25 μmol/L 和 50 μmol/L ) significantly inhibits HepG2 cell viability , but 10 μmol/L berberine doesn't .10 μmol/L berberine inhibits HepG2 cell migration in vitro and induces HepG2 cell autophagy .3-MA re-verses the effect of berberine on HepG2 cell migration .Conclusion :Berberine in a low concentration inhibits HepG2 cell migration in vitro via autophagy .%目的:探讨黄连素对肝癌细胞系HepG2细胞体外迁移的作用及其机制.方法:通过MTT实验,检测不同浓度的黄连素对HepG2细胞存活的影响.利用细胞划痕迁移实验,观察低浓度黄连素(10μmol/L)对HepG2细胞体外迁移的影响以及3-MA对黄连素调控HepG2细胞体外迁移作用的影响.采用Western blot实验,证实低浓度黄连素引起HepG2细胞发生自噬.结果:高浓度黄连素(25μmol/L和50μmol/L)显著抑制HepG2细胞存活,低浓度黄连素未见明显变化.低浓度黄连素抑制HepG2细胞体外迁移并且引起HepG2细胞发生自噬,而3-MA能够逆转黄连素抑制HepG2细胞迁移的作用.结论:低浓度黄连素通过自噬抑制HepG2细胞体外迁移.
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