Objective To investigate the effects of baicalein on proliferation, apoptosis and invasion of human color-ectal cancer cells and to explore the mechanism.Methods Human colorectal cancer cells SW480 in logarithmic phase were randomly divided into the control group, 25, 50 and 100 μmol/L baicalein treatment groups which were respectively treated with normal saline, 25, 50 and 100μmol/L baicalein.The effects of baicalein on proliferation, apoptosis and inva-sion of SW480 cells were detected by EdU, MTT, flow cytometry and Transwell test.The protein expression of Bcl-2, Bax and vascular endothelial growth factor ( VEGF) in SW480 cells after the treatment of baicalein was detected by Western blotting.Results In the 25, 50 and 100μmol/L baicalein treatment groups, the proliferation of SW480 cells was inhibited with a dose and time-dependent manner, the apoptosis rates of SW480 cells were gradually increased, and the invasion a-bilities of SW480 cells were gradually decreased.After the treatment of baicalein, the expression of Bcl-2 and VEGF in SW480 cells was down-regulated, and the expression of Bax was up-regulated (P<0.05).Conclusion Baicalein inhibits the proliferation and invasion of SW480 cells and promots the apoptosis, which might be related with the down-regulation of Bcl-2 and VEGF expression and the up-regulation of Bax expression.%目的：探讨黄芩素对人结肠癌细胞增殖、凋亡、侵袭的影响及其作用机制。方法取处于对数生长期的人结肠癌SW480细胞，随机分为对照组，25、50、100μmol／L黄芩素处理组，分别加入生理盐水，25、50、100μmol／L黄芩素处理，采用EdU、MTT、流式细胞术及Transwell小室实验观察黄芩素对结肠癌细胞增殖、凋亡及侵袭的影响，并通过Westernblotting法检测黄芩素干预后结肠癌细胞Bcl-2、Bax及VEGF蛋白表达。结果25、50、100μmol／L黄芩素处理组SW480细胞增殖受到抑制，呈剂量、时间依赖性；25、50、100μmol／L黄芩素处理组SW480细胞凋亡率依次升高；25、50、100μmol／L黄芩素处理组SW480细胞的侵袭迁移能力降低；黄芩素干预后SW480细胞中Bcl-2、VEGF蛋白表达下调，Bax蛋白表达上调（ P＜0．05）。结论黄芩素抑制人结肠癌细胞的增殖及侵袭，并促进其凋亡，机制可能与下调Bcl-2、VEGF蛋白表达并上调Bax蛋白表达有关。
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