Objective To establish a radioresistant xenograft tumor model of human nasopharyngeal carcinoma(NPC) and study its characteristics of growth, so as to supply a good model for investigating the mechanism of radioresistance. Methods Radioresistant human NPC CNE-2R cells and CNE-2 cells were subcutaneously injected into hind legs of nude mice. Diameters of subcutaneous xenograft tumors were measured with a caliper after the tumors were palpated, and vol-umes of the tumors were regularly calculated, then growth curve of the tumors was drawn. The tumors-bearing mice received X-ray radiation when the tumors reached 0. 8-1 cm in size, 16 Gy in 4 fractions. Tumor doubling time and inhibition of the volumes of the tumors were calculated. The mice were sacrificed two weeks after irradiation and the tumor tissues were ex-amined for histopathology. The expression of PCNA protein in xenograft tumors was detected by immunohistochemistry. Re-sults All the xenograft tumor models of CNE-2R cells and CNE-2 cells were established successfully. Compared to the CNE-2 tumors, the CNE-2R tumors grew significantly slowly, its growth was not inhibited by irradiation; and its PCNA lev-el was obviously lower. Conclusion The CNE-2R xenograft tumors are much radioresistant than the CNE-2 xenograft tumors, the established CNE-2R xenograft tumors model can be used as a reliable radioresistant NPC mouse model.%目的 建立稳定的人鼻咽癌放射抗拒移植瘤模型并观察其生长特性,为解决鼻咽癌放射抗拒提供良好的动物模型.方法 将人鼻咽癌放射抗拒细胞CNE-2R、人鼻咽癌细胞CNE-2分别注入裸鼠左右后肢皮下.移植瘤生长后,隔日测量肿瘤直径,计算肿瘤体积,绘制移植瘤体积生长曲线.肿瘤直径0.8～1 cm时以X线隔日照射移植瘤,1 600Gy分4次.计算移植瘤体积倍增时间和体积增长率.照射后2周取出移植瘤行常规病理检查,免疫组化检测增殖细胞核抗原(PCNA)的表达.结果 CNE-2R、CNE-2细胞的皮下成瘤率均为100％,建模成功.相对于CNE-2移植瘤,CNE-2R移植瘤生长速度慢,生长受照射抑制不明显；PCNA表达较低.结论 相对于CNE-2移植瘤,CNE-2R移植瘤具有更强的放射抗拒性,CNE-2R裸鼠移植瘤模型是进一步研究鼻咽癌放射抗拒的有力工具.
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