Objective To observe the methylation levels of P16 and hMLH1DNA genes in different TCM syndromes to find out the possible causes of the differences between them.Methods A questionnaire on TCM syndrome was designed according to the literature and the first edition of the Guidelines for Clinical Study of New Drugs on Chinese Medicines. 180 patients diagnosed by endoscopy with chronic atrophic gastritis were chosen based on the inclusion and exclusion criteria for TCM syndrome differentiation. Pyrophosphoric acid sequencing on 72 patients was used to detect the P16 & hMLH1 DNA methylation. Statistical analysis was done to determine the methylation levels of oncogenes,tumor suppressor genes as well as Mismatch repair genes and the correlation between them to determine the correlation between different TCM syndromes of chronic atrophic gastritis. Results The P16 DNA methylation rates in the syndromes were liver-stomach disharmony> deficiency-cold in spleen and stomach> deficiency of qi and blood> phlegm-dampness coagulation> stomach heat impairing yin> stasis toxin stagnation. The differences between the stomach heat impairing yin group and the stasis toxin stagnation group and the liver-stomach disharmony group were statistically significant(P<0.05).The hMLH1 DNA methylation rates were not significantly different between the TCM syndromes of chronic atrophic gastritis(P>0.05).Conclusion P16 DNA methylation shows statistically significant differences in different TCM syndromes of chronic atrophic gastritis.The hMLH1 DNA methylation rates are not significantly different between the TCM syndromes of chronic atrophic gastritis.%目的 通过观察P16、hMLH1 DNA甲基化水平在慢性萎缩性胃炎不同中医证型间的差异,探讨中医证型与抑癌基因、错配修复基因甲基化的关系.方法参照《中药新药临床研究指导原则》并在文献研究基础上设计中医证型问卷调查表.根据病例纳入标准,选取180例内镜诊断为慢性萎缩性胃炎患者进行中医辨证分型.采用焦磷酸测序法检测72例患者胃黏膜中的P16、hMLH1 DNA甲基化表达.统计分析不同中医证型胃黏膜抑癌基因、错配修复基因的DNA甲基化水平差异及其相关性.结果慢性萎缩性胃炎中医且各证型中p16基因甲基化率由高至低依次是肝胃不和>脾胃虚寒>气血双亏>痰湿凝结>胃热伤阴>瘀毒内阻.胃热伤阴、瘀毒内阻组与肝胃不和组比较,差异有统计学意义(P<0.05).hMLH1DNA基因甲基化率在慢性萎缩性胃炎中医各证型间比较差异无统计学意义(P>0.05).结论P16基因甲基化率与慢性萎缩性胃炎中医各证型有相关性.慢性萎缩性胃炎不同中医证型hMLH1DNA基因甲基化率不存在差异.
展开▼
