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首页> 美国卫生研究院文献>International Journal of Analytical Chemistry >A New HPLC-ELSD Method for Simultaneous Determination of N-Acetylglucosamine and N-Acetylgalactosamine in Dairy Foods
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A New HPLC-ELSD Method for Simultaneous Determination of N-Acetylglucosamine and N-Acetylgalactosamine in Dairy Foods

机译：HPLC-ELSD新方法同时测定乳制品中的N-乙酰氨基葡萄糖和N-乙酰半乳糖

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A rapid high performance liquid chromatographic method with evaporative light scattering detection (HPLC-ELSD), using a carbohydrate column, was developed for simultaneous determination of N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) in dairy foods. Sample preparation was performed by precipitation using acetonitrile. The limits of detection were 2.097 mg/L for GlcNAc and 3.247 mg/L for GalNAc. The limits of quantification were 6.043 mg/L for GlcNAc and 9.125 mg/L for GalNAc. Accuracy ranged from 96.4 to 105.7% for GlcNAc and from 97.1 to 104.1% for GalNAc. The precision of the method was <1.7% for GlcNAc and <2.2% for GalNAc. The mean recovery of the method was measured by spiking samples with 30.0–120.0 mg/L GlcNAc or 12.5–50.0 mg/L GalNAc and was found to be 95.1–105.5% for GlcNAc and 99.5–105.9% for GalNAc. The stability test results of standard solutions stored at 4, 20, and 40°C were 96.2–104.7% for GlcNAc and 98.0–106.5% for GalNAc. This study determined GlcNAc and GalNAc in dairy foods using HPLC-ELSD method. This rapid, simultaneous quantitation method might be useful as a mean of convenient quality control of dairy foods.
机译：建立了使用碳水化合物色谱柱的快速高效液相色谱-蒸发光散射检测（HPLC-ELSD）方法，用于同时测定乳制品中的N-乙酰氨基葡萄糖（GlcNAc）和N-乙酰半乳糖胺（GalNAc）。通过使用乙腈沉淀进行样品制备。 GlcNAc的检出限为2.097μg/ L，GalNAc的检出限为3.247μg/ L。 GlcNAc的定量限为6.043μmg/ L，GalNAc的定量限为9.125μmg/ L。 GlcNAc的准确度范围从96.4至105.7％，GalNAc的准确度范围从97.1至104.1％。该方法的精确度对于GlcNAc而言<1.7％，对于GalNAc而言<2.2％。该方法的平均回收率是通过加标30.0–120.0μmg / L GlcNAc或12.5–50.0μmg / L GalNAc的样品测得的，发现GlcNAc为95.1–105.5％，GalNAc为99.5–105.9％。在4、20和40°C下储存的标准溶液的稳定性测试结果，GlcNAc为96.2–104.7％，GalNAc为98.0–106.5％。本研究使用HPLC-ELSD方法测定了乳制品中的GlcNAc和GalNAc。这种快速，同时的定量方法可能作为便利控制乳制品质量的一种手段。

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