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首页> 美国卫生研究院文献>Mediators of Inflammation >Extraction Process Component Analysis and In Vitro Antioxidant Antibacterial and Anti-Inflammatory Activities of Total Flavonoid Extracts from Abutilon theophrasti Medic. Leaves
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Extraction Process Component Analysis and In Vitro Antioxidant Antibacterial and Anti-Inflammatory Activities of Total Flavonoid Extracts from Abutilon theophrasti Medic. Leaves

机译：Ab麻中总黄酮提取物的提取工艺成分分析和体外抗氧化抗菌和抗炎活性。树叶

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The flavonoid fraction was extracted from the leaves of Abutilon theophrasti Medic., which are usually used as a traditional Chinese herbal medicine for the treatment of inflammation and joint pain. The current study focused on the extraction process, component analysis, and in vitro antioxidant, antibacterial, and anti-inflammatory activities of the flavonoid fraction as a part of ongoing research on bioactive substances from natural plant sources. This study evaluated the antioxidant activities via assays of DPPH radical scavenging capacity, ABTS radical scavenging capacity, and reducing power and investigated inhibitory activities against Escherichia coli, Salmonella, Staphylococcus aureus, and Streptococcus. Moreover, the inflammatory activity of the flavonoid fraction was estimated by measurement of the content of tumor necrosis factor alpha, interleukin-1-beta, interleukin-6, interleukin-10, nitric oxide, and cyclooxygenase-2 and the gene expression levels of several inflammation markers, such as inducible nitric oxide synthase and cyclooxygenase-2, in RAW 264.7 macrophages after LPS treatment. In addition, the underlying anti-inflammatory mechanisms, that is, the activation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways, were also revealed from the gene and protein expression levels. Taken together, these results suggested that the flavonoid fraction might exert in vitro antioxidant, antibacterial, and anti-inflammatory effects on LPS-stimulated RAW 264.7 macrophages and will be potentially useful as an adjuvant treatment for oxidative stress and bacterial and inflammatory diseases.
机译：黄酮类成分是从白屈菜的叶中提取的，该叶通常用作治疗炎症和关节痛的传统中草药。目前的研究集中在黄酮类成分的提取过程，成分分析和体外抗氧化，抗菌和抗炎活性，这是对天然植物来源生物活性物质进行研究的一部分。这项研究通过测定DPPH自由基清除能力，ABTS自由基清除能力和还原能力来评估抗氧化活性，并研究了其对大肠杆菌，沙门氏菌，金黄色葡萄球菌和链球菌的抑制活性。此外，通过测量肿瘤坏死因子α，白介素-1-β，白介素-6，白介素-10，一氧化氮和环氧合酶-2的含量以及几种基因的表达水平来评估类黄酮部分的炎性活性。 LPS处理后RAW 264.7巨噬细胞中的炎症标志物，例如诱导型一氧化氮合酶和环氧合酶-2。此外，还从基因和蛋白质表达水平揭示了潜在的抗炎机制，即核因子κB（NF-κB）和有丝分裂原激活的蛋白激酶（MAPK）信号通路的激活。综上所述，这些结果表明类黄酮部分可能对LPS刺激的RAW 264.7巨噬细胞发挥体外抗氧化，抗菌和抗炎作用，并可能潜在地用作氧化应激以及细菌性和炎性疾病的辅助治疗。

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