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首页> 美国卫生研究院文献>Molecular and Cellular Biology >Six mouse alpha-tubulin mRNAs encode five distinct isotypes: testis-specific expression of two sister genes.
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Six mouse alpha-tubulin mRNAs encode five distinct isotypes: testis-specific expression of two sister genes.

机译：六个小鼠α-微管蛋白mRNA编码五个不同的同种型：两个姐妹基因的睾丸特异性表达。

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摘要

Five mouse alpha-tubulin isotypes are described, each distinguished by the presence of unique amino acid substitutions within the coding region. Most, though not all of these isotype-specific amino acids, are clustered at the carboxy terminus. One of the alpha-tubulin isotypes described is expressed exclusively in testis and is encoded by two closely related genes (M alpha 3 and M alpha 7) which have homologous 3' untranslated regions but which differ at multiple third codon positions and in their 5' untranslated regions. We show that a subfamily of alpha-tubulin genes encoding the same testis-specific isotype also exists in humans. Thus, we conclude that the duplication event leading to a pair of genes encoding a testis-specific alpha-tubulin isotype predated the mammalian radiation, and both members of the duplicated sequence have been maintained since species divergence. A second alpha-tubulin gene, M alpha 6, is expressed ubiquitously at a low level, whereas a third gene, M alpha 4, is unique in that it does not encode a carboxy-terminal tyrosine residue. This gene yields two transcripts: a 1.8-kilobase (kb) mRNA that is abundant in muscle and a 2.4-kb mRNA that is abundant in testis. Whereas the 1.8-kb mRNA encodes a distinct alpha-tubulin isotype, the 2.4-kb mRNA is defective in that the methionine residue required for translational initiation is missing. Patterns of developmental expression of the various alpha-tubulin isotypes are presented. Our data support the view that individual tubulin isotypes are capable of conferring functional specificity on different kinds of microtubules.
机译：描述了五个小鼠α-微管蛋白同种型，每个同种型的区别在于编码区内存在独特的氨基酸取代。尽管不是所有这些同种型特异性氨基酸，但大多数（但不是全部）聚集在羧基末端。所述的一种α-微管蛋白同种型仅在睾丸中表达，并由两个紧密相关的基因（M alpha 3和M alpha 7）编码，这些基因具有同源的3'非翻译区，但在多个第三密码子位置及其5'处不同未翻译的区域。我们表明，在人类中也存在编码相同睾丸特异性同种型的α-微管蛋白基因的亚家族。因此，我们得出结论，导致一对基因编码睾丸特异的α-微管蛋白同种型的重复事件早于哺乳动物辐射，并且自物种分化以来，一直保持重复序列的两个成员。第二个α-微管蛋白基因M alpha 6以低水平普遍表达，而第三个基因Mα4独特，因为它不编码羧基末端的酪氨酸残基。该基因产生两个转录本：一个在肌肉中丰富的1.8碱基对（kb）mRNA和一个在睾丸中丰富的2.4 kb mRNA。尽管1.8-kb mRNA编码独特的α-微管蛋白同种型，但2.4-kb mRNA却有缺陷，因为缺少翻译起始所需的甲硫氨酸残基。介绍了各种α-微管蛋白同种型的发育表达模式。我们的数据支持这样的观点，即单个微管蛋白同种型能够赋予不同种类的微管功能特异性。

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