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首页> 外文期刊>Molecular and Cellular Biology >Six mouse alpha-tubulin mRNAs encode five distinct isotypes: testis-specific expression of two sister genes.
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Six mouse alpha-tubulin mRNAs encode five distinct isotypes: testis-specific expression of two sister genes.

机译：六只小鼠α-小管蛋白mRNA编码五个不同的同种型：睾丸特异性的两个姐妹基因的表达。

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Five mouse alpha-tubulin isotypes are described, each distinguished by the presence of unique amino acid substitutions within the coding region. Most, though not all of these isotype-specific amino acids, are clustered at the carboxy terminus. One of the alpha-tubulin isotypes described is expressed exclusively in testis and is encoded by two closely related genes (M alpha 3 and M alpha 7) which have homologous 3' untranslated regions but which differ at multiple third codon positions and in their 5' untranslated regions. We show that a subfamily of alpha-tubulin genes encoding the same testis-specific isotype also exists in humans. Thus, we conclude that the duplication event leading to a pair of genes encoding a testis-specific alpha-tubulin isotype predated the mammalian radiation, and both members of the duplicated sequence have been maintained since species divergence. A second alpha-tubulin gene, M alpha 6, is expressed ubiquitously at a low level, whereas a third gene, M alpha 4, is unique in that it does not encode a carboxy-terminal tyrosine residue. This gene yields two transcripts: a 1.8-kilobase (kb) mRNA that is abundant in muscle and a 2.4-kb mRNA that is abundant in testis. Whereas the 1.8-kb mRNA encodes a distinct alpha-tubulin isotype, the 2.4-kb mRNA is defective in that the methionine residue required for translational initiation is missing. Patterns of developmental expression of the various alpha-tubulin isotypes are presented. Our data support the view that individual tubulin isotypes are capable of conferring functional specificity on different kinds of microtubules.
机译：描述了五种小鼠α-微管蛋白同种型，各自通过编码区内的独特氨基酸取代的存在而区分。最多，尽管不是所有这些异型特异性氨基酸，都在羧基末端聚集在羧基末端。所描述的一种α-微管蛋白同种型仅在睾丸中表达，并且由两个密切相关的基因（Mα3和M alpha 7）编码，其具有同源3'未翻译的区域，但在多个第三个密码子位置和它们的5'中不同。未经翻译的地区。我们表明，在人类中存在编码相同的睾丸特异性同种型的α-微管蛋白基因的亚家族。因此，我们得出结论，导致编码睾丸特异性α-微管蛋白同种型的一对基因的复制事件捕获哺乳动物辐射，并且由于物种分歧，因此已经保持了重复序列的两种成员。第二α-微管蛋白基因Mα6在低水平下普遍地表达，而第三基因Mα4是独特的，因为它不会编码羧基末端酪氨酸残基。该基因产生两种转录物：1.8千碱基（KB）mRNA，肌肉丰富，睾丸丰富的2.4kb mRNA。然而，1.8-kB mRNA编码不同的α-微管蛋白同种型，2.4kb mRNA有缺陷，因为翻译起始所需的甲硫氨酸残基缺失。提出了各种α-微管蛋白同种型的发育表达的模式。我们的数据支持该视图，即单个微管蛋白同种型能够在不同种类的微管上赋予功能特异性。

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