Antigen-dependent in vitro culture of protective T cells from BCG-primed mice.

机译：来自BCG致敏小鼠的保护性T细胞的抗原依赖性体外培养。

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Induction of protective immunity against pathogenic mycobacteria depends on vaccination with live organisms such as Bacille Calmette-Guérin (BCG). However, it is not known how many and which antigens are involved in the protective host response. In this study, we developed a system of antigen-dependent in vitro culture which is suitable for the analysis of protective subunits, presented in a soluble form. Spleen cells from Mycobacterium bovis BCG-immune mice, enriched for T cells and depleted of adherent cells on a column of G-10 Sephadex, were cultured for periods varying between 3 and 14 days before transfer and challenge with M. tuberculosis in irradiated hosts. Following 10 days in culture, immune T cells sustained their capacity to transfer protection to tuberculous infection when incubated in the presence of either live BCG or a soluble extract from M. tuberculosis, but lost this ability when cultured in the absence of antigen, or in the presence of the polyclonal mitogen concanavalin A. One immunodominant antigen, represented by the recombinant 38-kD antigen, failed to sustain the adoptive protection, despite pronounced stimulation of lymphoproliferation in culture. Antigenic in vitro stimulation of protective T cells was accompanied by enhanced responsiveness to exogenous IL-2. The experimental system described may be generally suitable to test in vitro the protective potentials of soluble molecular subunits of mycobacteria.

机译：对致病性分枝杆菌的保护性免疫的诱导取决于对活生物体（如巴克·卡莱梅特·盖林（BCG））的接种。然而，尚不知道保护性宿主应答中涉及多少种抗原以及哪些抗原。在这项研究中，我们开发了一种抗原依赖的体外培养系统，适用于分析以可溶性形式存在的保护性亚基。将来自牛分枝杆菌BCG免疫小鼠的脾细胞富集T细胞并去除G-10 Sephadex色谱柱上的贴壁细胞，然后在转移和接受辐照宿主的结核分枝杆菌攻击之前培养3至14天。培养10天后，在活的BCG或结核分枝杆菌的可溶性提取物存在下孵育时，免疫T细胞仍具有将保护转移至结核感染的能力，但在没有抗原或在无抗原的条件下培养时，则失去了这种能力。尽管明显刺激了培养物中的淋巴细胞增殖，但以重组38-kD抗原为代表的一种免疫优势抗原未能维持过继性保护作用。抗原性保护性T细胞的体外刺激伴随着对外源IL-2的增强反应性。所述的实验系统通常可以适合于体外测试分枝杆菌的可溶性分子亚基的保护潜力。

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期刊名称 Clinical and Experimental Immunology
作者
S DSouza; J Ivanyi;
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年(卷),期 1993(91),1
年度 1993
页码 68–72
总页数 5
原文格式 PDF
正文语种
中图分类免疫学;
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专利

1. Identification of a B-cell surface structure involved in antigen-dependent triggering: absence of this structure on B cells from CBA/N mutant mice. [J] . B Huber, R K Gershon, H Cantor The Journal of Experomental Medicine . 1977,第1期

机译：鉴定涉及抗原依赖性触发的B细胞表面结构：来自CBA / N突变小鼠的B细胞上没有这种结构。
2. In vitro studies on lymphocyte differentiation. I. Long term in vitro culture of cells giving rise to functional lymphocytes in irradiated mice. [J] . J W Schrader, S Schrader The Journal of Experomental Medicine . 1978,第3期

机译：淋巴细胞分化的体外研究。 I.在辐射小鼠中长期体外培养细胞产生功能性淋巴细胞。
3. Antigen-dependent IgM-mediated enhancement of the sheep erythrocyte response in mice. Evidence for induction of B cells with specificities other than that of the injected antibodies [J] . B Heyman, G Andrighetto, H Wigzell The Journal of Experomental Medicine . 1982,第4期

机译：抗原依赖性IgM介导的小鼠绵羊红细胞应答增强。诱导B细胞具有不同于所注射抗体的特异性的证据
4. Elucidation of Neuroprotective Properties of Astrocytoma (Astrocytes-like) Cells in Neural Cell Culture Models In Vitro: Applications in Tissue Engineering and Nanotoxicology [C] . Y.Y.W. Wong, A.R. Jaiswal, V.V. Dukhande, NSTI-Nanotech 2010;NSTI nanotechnology conference and expo;Bio Nanotech 2010;NSTI bio nano conference and expo;Microtech 2010;Microtechnology conference and expo;WCM 2010;Workshop on compact modeling;NSTI nanotech ventures;TechConnect summit;Clean technology . 2010

机译：阐明神经细胞培养模型中星形细胞瘤（星形胶质细胞）细胞的神经保护特性：在组织工程和纳米毒理学中的应用
5. Using a Real-Time Cellular Biosensor to Investigate in vitro Liver Culture Models; Exploring the Effect of Primary Cultures, Cell to Cell Interaction in Co-culture, and Cell to Extracellular Matrix Interactions [D] . Ciampa, Grace. 2019

机译：使用实时细胞生物传感器来研究体外肝培养模型;探讨原发性培养物，细胞对共培养细胞相互作用的影响，以及细胞对细胞基质相互作用
6. BCG Corynebacterium parvum or Mycobacterium leprae added to cultures of BCG-primed mouse spleen cells cause an enhanced primary antibody response in vitro. [O] . C A Brown, I N Brown, V S Sljivić 1981

机译：添加到BCG引发的小鼠脾细胞培养物中的BCG小棒杆菌或麻风分枝杆菌会增强体外的一抗反应。
7. Antigen-dependent in vitro culture of protective T cells from BCG-primed mice. [O] . D'Souza, S, Ivanyi, J 2008

机译：来自BCG致敏小鼠的保护性T细胞的抗原依赖性体外培养。
8. In vitro Antigen-Specific Antibody Response to the Species-Specific Surface Protein Antigens of Typhus Group Rickettsiae by Human Peripheral Blood Mononuclear Cells. Generation of an Antigen-Dependent Suppressor T Cell [R] . Misiti, J., Dasch, G. A. 1985

机译：体外抗原特异性抗体对人外周血单个核细胞对斑疹伤寒组立克次体的物种特异性表面蛋白抗原的反应。产生抗原依赖性抑制T细胞

Antigen-dependent in vitro culture of protective T cells from BCG-primed mice.

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