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Overexpression of STARCH BRANCHING ENZYME II increases short-chain branching of amylopectin and alters the physicochemical properties of starch from potato tuber

机译：过表达STARCH分支酶II会增加支链淀粉的短链分支并改变马铃薯块茎淀粉的理化特性

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BackgroundStarch is biosynthesised by a complex of enzymes including various starch synthases and starch branching and debranching enzymes, amongst others. The role of all these enzymes has been investigated using gene silencing or genetic knockouts, but there are few examples of overexpression due to the problems of either cloning large genomic fragments or the toxicity of functional cDNAs to bacteria during cloning. The aim of this study was to investigate the function of potato STARCH BRANCHING ENZYME II (SBEII) using overexpression in potato tubers.

机译：背景技术淀粉是由多种酶合成的，包括多种淀粉合酶，淀粉分支和脱支酶等。已经使用基因沉默或基因敲除研究了所有这些酶的作用，但是由于克隆大基因组片段或功能性cDNA对细菌的毒性问题，很少有过表达的例子。这项研究的目的是利用马铃薯块茎中的过表达研究马铃薯STARCH分支酶II（SBEII）的功能。

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期刊名称 BMC Biotechnology
作者
David A Brummell; Lyn M Watson; Jun Zhou; Marian J McKenzie; Ian C Hallett; Lyall Simmons; Margaret Carpenter; Gail M Timmerman-Vaughan;
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作者单位

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年(卷),期 2015(15),-1
年度 2015
页码 28
总页数 14
原文格式 PDF
正文语种
中图分类应用微生物学;
关键词
Amylopectin branching GBSS silencing SBEII overexpression Starch gelatinisation Starch properties Solanum tuberosum;

机译：支链淀粉分支;GBSS沉默;SBEII过表达;淀粉糊化;淀粉特性;马铃薯;

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专利

1. Maize starch-branching enzyme isoforms and amylopectin structure. In the absence of starch-branching enzyme IIb, the further absence of starch-branching enzyme ia leads to increased branching [J] . Yao Y, Thompson DB, Guiltinan MJ Plant physiology . 2004,第3期

机译：玉米淀粉分支酶同工型和支链淀粉的结构。在不存在淀粉分支酶IIb的情况下，进一步不存在淀粉分支酶ia导致分支增加
2. Starch phosphorylation in potato tubers is influenced by allelic variation in the genes encoding glucan water dikinase, starch branching enzymes I and II, and starch synthase III [J] . Margaret A. Carpenter, Nigel I. Joyce, Russell A. Genet, Frontiers in Plant Science . 2015,第1期

机译：马铃薯块茎中的淀粉磷酸化受到编码葡聚糖水二激酶，淀粉分支酶I和II和淀粉合酶III的基因的等位基因变异的影响
3. Inhibition of starch branching enzymes in waxy rice increases the proportion of long branch-chains of amylopectin resulting in the comb-like profiles of starch granules [J] . He Wei, Lin Lingshang, Wang Juan, Plant Science: An International Journal of Experimental Plant Biology . 2018,第期

机译：蜡质水稻中淀粉分枝酶的抑制增加了支链淀粉的长分支链的比例，得到淀粉颗粒的梳状曲线
4. Effect of starch-branching enzyme lib on amylopectin structure and gelatinization property [C] . A. Nishi, Y. Nakamura, H. Satoh International Rice Genetics Symposium . 2008

机译：淀粉分支酶Lib对淀粉素结构和凝胶化性能的影响
5. Structure and function of endosperm starch from maize mutants deficient in one or more starch-branching enzyme isoform activities. [D] . Xia, Huan. 2009

机译：缺乏一种或多种淀粉分支酶同工型活性的玉米突变体的胚乳淀粉的结构和功能。
6. Maize Starch-Branching Enzyme Isoforms and Amylopectin Structure. In the Absence of Starch-Branching Enzyme IIb, the Further Absence of Starch-Branching Enzyme Ia Leads to Increased Branching [O] . Yuan Yao, Donald B. Thompson, Mark J. Guiltinan 2018

机译：玉米淀粉分支酶同工型和支链淀粉结构。在缺乏淀粉分支酶IIb的情况下，进一步缺乏淀粉分支酶Ia会导致分支增加
7. Overexpression of increases short-chain branching of amylopectin and alters the physicochemical properties of starch from potato tuber [O] . 2015

机译：过表达增加支链淀粉的短链分支并改变马铃薯块茎淀粉的理化特性
8. Molecular Genetic Analysis of Maize Starch Branching Isoforms: Modulation of Starch Branching Enzyme Isoform Activities in Maize to Produce Starch with Novel Branching Architecture and Properties [R] . Guiltinan, M. J., Thompson, D. 2008

机译：玉米淀粉分支异构体的分子遗传分析：玉米淀粉分支酶同工酶活性调控产生淀粉的新分支结构和性质

Overexpression of STARCH BRANCHING ENZYME II increases short-chain branching of amylopectin and alters the physicochemical properties of starch from potato tuber

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