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首页> 外文期刊>Plant Science: An International Journal of Experimental Plant Biology >Inhibition of starch branching enzymes in waxy rice increases the proportion of long branch-chains of amylopectin resulting in the comb-like profiles of starch granules
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Inhibition of starch branching enzymes in waxy rice increases the proportion of long branch-chains of amylopectin resulting in the comb-like profiles of starch granules

机译：蜡质水稻中淀粉分枝酶的抑制增加了支链淀粉的长分支链的比例，得到淀粉颗粒的梳状曲线

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摘要

Starches with comb-like profiles have been detected in some cereal endosperms with inhibiting expression of starch branching enzyme (SBE). Although amylose is considered to be an important factor in the formation of the comb-like profile, the details remain unclear. In this study, a transgenic rice line (GLXN-SBEI/IIb(-)) was derived from japonica waxy rice cultivar Guang-ling-xiang-nuo (GLXN) through antisense RNA inhibition of both SBEI and SBEIIb. The expression and activity of SBEI, SBEIIb and SBEIIa were declined. The GLXN-SBEI/IIb(-) endosperm contained large and small starch granules, and these starch granules had the comb-like profiles. The comb-like profiles of starches were detected in GLXN-SBEI/IIb(-) endosperm after 10 days after flowering with gradually increasing proportion of long branch-chains of amylopectin. The long branch-chains of amylopectin were responsible for forming the comb-like profiles at the outer region of starch granules. The gradually decreasing expression of SBEs influenced the synthesis of amylopectin during endosperm development, resulting in different structure between the inner and outer regions of starch granules from GLXN-SBEI/IIb(-) endosperm. The above results indicated that the long branch-chains of amylopectin, not amylose, led to the formation of comb like profiles of starch granules in cereal crops with inhibiting expression of SBEs.
机译：在一些谷物胚乳中检测到具有梳状型材的淀粉，其具有抑制淀粉分支酶（SBE）的表达。虽然直链淀粉被认为是形成梳状轮廓的重要因素，但细节仍然不清楚。在本研究中，通过反义RNA抑制斯巴西和Sbeiib的反义RNA抑制，衍生自粳稻（Glxn-Sbei / Iib（ - ））衍生自粳稻蜡质水稻品种（Glxn）。斯巴i，sbeiib和sbeiia的表达和活性被下降。 GLXN-SBEI / IIB（ - ）胚乳含有大而小的淀粉颗粒，并且这些淀粉颗粒具有梳状型材。在开花后10天后在GLXN-SBEI / IIB（ - ）胚乳中检测到淀粉的梳状曲线，逐渐增加了支链淀粉的长分支链的比例。支链淀粉的长分支链负责在淀粉颗粒的外部区域形成梳状曲线。逐渐降低的SBE表达影响了胚乳显影过程中的淀粉型素的合成，导致来自GLXN-SBEI / IIB（ - ）胚乳的淀粉颗粒的内部和外部区域之间的不同结构。上述结果表明，支链淀粉的长分支链，而不是淀粉糖，导致形成谷物颗粒在谷物作物中的梳状曲线，其具有抑制SBE的表达。

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  • 作者

    He Wei; Lin Lingshang; Wang Juan; Zhang Long; Liu Qiaoquan; Wei Cunxu;

  • 作者单位

    Yangzhou Univ Key Lab Crop Genet &

    Physiol Jiangsu Prov Key Lab Plant Funct Genom Minist Educ Yangzhou 225009 Jiangsu Peoples R China;

    Yangzhou Univ Key Lab Crop Genet &

    Physiol Jiangsu Prov Key Lab Plant Funct Genom Minist Educ Yangzhou 225009 Jiangsu Peoples R China;

    Yangzhou Univ Key Lab Crop Genet &

    Physiol Jiangsu Prov Key Lab Plant Funct Genom Minist Educ Yangzhou 225009 Jiangsu Peoples R China;

    Yangzhou Univ Key Lab Crop Genet &

    Physiol Jiangsu Prov Key Lab Plant Funct Genom Minist Educ Yangzhou 225009 Jiangsu Peoples R China;

    Yangzhou Univ Key Lab Crop Genet &

    Physiol Jiangsu Prov Key Lab Plant Funct Genom Minist Educ Yangzhou 225009 Jiangsu Peoples R China;

    Yangzhou Univ Key Lab Crop Genet &

    Physiol Jiangsu Prov Key Lab Plant Funct Genom Minist Educ Yangzhou 225009 Jiangsu Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物学;
  • 关键词

    Waxy rice; Starch branching enzyme; Expression inhibition; Comb-like profile of starch granule; Amylopectin;

    机译：蜡质米;淀粉分支酶;表达抑制;淀粉颗粒的梳状轮廓;淀粉蛋白;

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