We demonstrate the ability of a multimode fiber probe to provide two-photon fluorescence (TPF) imaging feedback that guides the femtosecond laser ablation (FLA) in biological samples for highly selective modifications. We implement the system through the propagation of high power femtosecond pulses through a graded-index (GRIN) multimode fiber and we investigate the limitations posed by the high laser peak intensities required for laser ablation. We demonstrate that the GRIN fiber probe can deliver laser intensities up to 1.5x1013 W/cm2, sufficient for the ablation of a wide range of materials, including biological samples. Wavefront shaping through an ultrathin probe of around 400 μm in diameter is used for diffraction limited focusing and digital scanning of the focus spot. Selective FLA of cochlear hair cells is performed based on the TPF images obtained through the same multimode fiber probe.
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机译：我们证明了多模光纤探针能够提供双光子荧光（TPF）成像反馈的能力，该反馈可指导飞秒激光消融（FLA）在生物样品中进行高度选择性的修饰。我们通过梯度折射率（GRIN）多模光纤传播高功率飞秒脉冲来实现该系统，并研究了激光烧蚀所需的高激光峰值强度所带来的局限性。我们证明，GRIN光纤探头可提供高达1.5x10 13 W / cm 2 的激光强度，足以烧蚀包括生物样品在内的各种材料。通过直径约400μm的超薄探头进行波前整形，用于衍射受限聚焦和焦点的数字扫描。耳蜗毛细胞的选择性FLA是基于通过同一多模光纤探针获得的TPF图像执行的。
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