• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Asian Pacific Journal of Cancer Prevention : APJCP >p16INK4a Expression in Cervical Lesions Correlates with Histologic Grading - a Tertiary Level Medical Facility Based Retrospective Study
【2h】

p16INK4a Expression in Cervical Lesions Correlates with Histologic Grading - a Tertiary Level Medical Facility Based Retrospective Study

机译：宫颈病变中p16INK4a表达与组织学分级相关-基于三级医疗设施的回顾性研究

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

p16INK4a is a tumor-suppressor protein and cyclin-dependent kinase (cdk) inhibitor that blocks cdk4- and cdk6-mediated pRb phosphorylation to inhibit E2F-dependent transcription and cell-cycle progression. Because the E7 protein of high-risk HPVs inactivates pRB, the resulting overexpression of p16INK4a may be a good marker for infection with high risk HPV types. Immunostaining of p16INK4a allows precise identification of even small CIN or cervical cancer lesions in biopsy sections and can help reduce inter-observer variation in the histopathological interpretation of cervical biopsy specimens. The aims of the present study were to evaluate the expression of p16 INK4a in cervical biopsies and to compare the grade of cervical neoplasia with intensity of staining. The study covered 110 cervical biopsy tissue blocks over a period of 2 years, (85 cases of CIN of varying grade and invasive cervical cancers and 25 of non-neoplastic lesions). Immunostaining with p16INK4a antibodies followed standard operating procedures. The results showed an increasing trend for p16INK4a immunoreactivity from benign to higher grade lesions. Out of 25 cases of non dysplasia (15 cervicitis &10 immature squamous metaplasia), 8%(2/25) showed P16INK4a expression (grade 1). Among low grade lesions like CIN1, 32% (8/25) cases demonstrated P16INK4a expression (grade 1). Some 52.3% (11/21) of CIN2 cases proved positive. The intensity of p16INK4a expression in CIN 2 was grade 1 in 33%, grade 2 in 14% and grade 3 in 4.7% of cases. All the CIN3 lesions and cervical squamous cell carcinomas exhibited grade 3 anti p16INK4a antibody staining. The association of p16INK4a expression with histologic grade of cervical pathology was highly significant (χ2-value:51.81, p<0.0001). The staining intensity increase with higher grade disease was also statistically significant (χ2-value :133.95, p<0.0001).
机译：p16INK4a是一种肿瘤抑制蛋白和细胞周期蛋白依赖性激酶（cdk）抑制剂，可阻断cdk4和cdk6介导的pRb磷酸化，从而抑制E2F依赖性转录和细胞周期进程。由于高危HPV的E7蛋白可灭活pRB，因此p16INK4a的过度表达可能是感染高危HPV类型的良好标志。 p16INK4a的免疫染色可在活检切片中精确识别甚至微小的CIN或宫颈癌病变，并有助于减少宫颈活检标本在组织病理学解释中的观察者间差异。本研究的目的是评估宫颈活检组织中p16 INK4a的表达，并比较宫颈癌的发展程度和染色强度。该研究在2年内涵盖了110例宫颈活检组织块（85例不同等级和浸润性宫颈癌的CIN和25例非肿瘤性病变）。按照标准操作程序对p16INK4a抗体进行免疫染色。结果表明，p16INK4a免疫反应性从良性病变到高级别病变的趋势不断增加。在25例非典型增生（15例宫颈炎和10例不成熟鳞状化生）中，有8％（2/25）表现出P16INK4a表达（1级）。在像CIN1这样的低度病变中，有32％（8/25）的病例表现出P16INK4a表达（1级）。大约52.3％（11/21）的CIN2病例证明是阳性。 CIN 2中p16INK4a表达的强度分别为1级（33％），2级（14％）和3级（4.7％）。所有CIN3病变和宫颈鳞状细胞癌均显示3级抗p16INK4a抗体染色。 p16INK4a表达与宫颈病理组织学分级的相关性极高（χ 2 值：51.81，p <0.0001）。较高等级疾病的染色强度增加也具有统计学意义（χ 2 值：133.95，p <0.0001）。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号