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首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Effects of Temperature Water Activity and Incubation Time on Production of Aflatoxins and Cyclopiazonic Acid by an Isolate of Aspergillus flavus in Surface Agar Culture
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Effects of Temperature Water Activity and Incubation Time on Production of Aflatoxins and Cyclopiazonic Acid by an Isolate of Aspergillus flavus in Surface Agar Culture

机译：温度水分活度和孵育时间对表面琼脂培养物中黄曲霉分离物生产黄曲霉毒素和环戊二酸的影响

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摘要

An experiment with a full factorial design was used to study the effects of and interactions among temperature, water activity (a(infw)), incubation period, and substrate on coproduction of aflatoxins (AF) and cyclopiazonic acid (CPA) by an isolate of Aspergillus flavus. Analysis of variance showed that there was a complex interaction among all of these factors and that this influenced the relative concentrations of the mycotoxins produced. The optimum temperatures for the production of AF and CPA were 30(deg)C and 25(deg)C, respectively. Both mycotoxins were maximally produced (0.306 to 0.330 (mu)g of AF(middot)ml of medium(sup-1), 4.040 to 6.256 (mu)g of CPA(middot)ml of medium(sup-1)) at an a(infw) of 0.996 and after 15 days of incubation. No AF were produced in either yeast extract agar or Czapek yeast autolysate agar medium at an a(infw) of 0.90 at 20 or 37(deg)C after 15 days (minimum conditions), while 0.077 to 0.439 (mu)g of CPA(middot)ml of medium(sup-1) was produced under the same conditions. Yeast extract agar favored maximum AF production, and Czapek yeast autolysate agar favored maximum CPA production.
机译：采用全因子设计的实验研究了温度，水分活度（a（infw）），潜伏期和底物对黄曲霉毒素（AF）和环吡唑酸（CPA）的分离产生的影响及其相互作用。黄曲霉。方差分析表明，所有这些因素之间存在复杂的相互作用，并且这影响所产生的霉菌毒素的相对浓度。用于生产AF和CPA的最佳温度分别为30℃和25℃。两种霉菌毒素的最高产量分别为（0.306至0.330μgAF（mid）ml培养基（sup-1），4.040至6.256μgCPA（midml）培养基（sup-1））。培养15天后的a（infw）为0.996。酵母提取琼脂或Czapek酵母自溶琼脂培养基在15天（最低条件）下在20或37℃下的a（infw）为0.90时，均未产生AF，而CPA（0.077至0.439μg在相同条件下产生了中等浓度的培养基（sup-1）ml。酵母提取物琼脂有利于最大的AF产量，而Czapek酵母自溶产物琼脂有利于最大的CPA产量。

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