首页> 美国卫生研究院文献>Applied and Environmental Microbiology >Modification of Sorbitol MacConkey Medium Containing Cefixime and Tellurite for Isolation of Escherichia coli O157:H7 from Radish Sprouts
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Modification of Sorbitol MacConkey Medium Containing Cefixime and Tellurite for Isolation of Escherichia coli O157:H7 from Radish Sprouts

机译:含头孢克肟和亚碲酸盐的山梨醇MacConkey培养基的修饰用于从萝卜芽中分离大肠杆菌O157:H7

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摘要

A modified version of sorbitol MacConkey medium containing cefixime and tellurite (CT-SMAC medium) was produced by adding salicin and 4-methylumbelliferyl-β-d-galactopyranoside to CT-SMAC medium; this medium was designated CT-SSMAC medium and was used to isolate Escherichia coli O157:H7 from radish sprouts. Of 101 non-E. coli bacteria isolated from radish sprouts that produced colorless colonies similar to colonies of E. coli O157:H7 grown on CT-SMAC medium, 92 (91%) formed colonies that were red to pink or were β-galactosidase negative and colorless on CT-SSMAC medium. On the other hand, colonies of E. coli O157:H7 strains were colorless and β-galactosidase positive on CT-SSMAC medium. Our results suggest that CT-SSMAC medium is more selective than CT-SMAC medium for isolating E. coli O157:H7.
机译:通过向CT-SMAC培养基中添加水杨素和4-甲基伞形酮基-β-d-吡喃半乳糖苷,制得含有头孢克肟和亚碲酸盐的山梨糖醇MacConkey培养基的改良版(CT-SMAC培养基)。将该培养基命名为CT-SSMAC培养基,用于从萝卜芽中分离大肠杆菌O157:H7。的101非E。从萝卜芽中分离出的大肠杆菌产生的无色菌落类似于在CT-SMAC培养基上生长的大肠杆菌O157:H7的菌落,其中92个(91%)形成的菌落呈红色至粉红色,或在β-半乳糖苷酶上呈β-半乳糖苷酶阴性和无色SSMAC介质。另一方面,大肠杆菌O157:H7菌株的菌落是无色的,并且在CT-SSMAC培养基上呈β-半乳糖苷酶阳性。我们的结果表明,CT-SSMAC培养基比CT-SMAC培养基对分离大肠杆菌O157:H7更具选择性。

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