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首页> 美国卫生研究院文献>Experimental and Therapeutic Medicine >Multicomponent quantification of Astragalus residue fermentation liquor using ion chromatography-integrated pulsed amperometric detection
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Multicomponent quantification of Astragalus residue fermentation liquor using ion chromatography-integrated pulsed amperometric detection

机译：离子色谱-积分脉冲安培检测法对黄芪残渣发酵液进行多组分定量分析

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摘要

Chinese medicine residues contain abundant cellulose and hemicellulose, which are potential renewable carbon sources for ethanol production. The aim of the present study was to develop a rapid and reliable method to evaluate the cellulose and hemicellulose utilization in Chinese medicine residues. In the present study, key hydrolysates (arabinose, galactose, glucose, xylose, and cellobiose) of the cellulose and hemicellulose in fermentation liquor of Astragalus residues were simultaneously quantified by ion chromatography using an integrated pulsed amperometric detector (IPAD). HPLC analysis was performed on a Dionex ICS-2500 equipped with GP50 gradient pump and ED50 IPAD. The working and reference electrodes were gold electrode and Ag/AgCl electrode, respectively. Separation was achieved on serial no. 002627 Dionex Analytical column (2×250 mm). Sodium hydroxide of 250 mM and water were used as the mobile phase with a flow rate of 0.2 ml/min. The temperature of column was kept at 30°C. This method was validated for accuracy and precision. The regression equation revealed a good linear relationship (R2=0.9959–0.9984) within the test ranges. The limits of detection and quantification for five standard analytes (arabinose, galactose, glucose, xylose and cellobiose) were in the range of 0.067–0.091 and 0.08–0.23 mg/l, respectively. The method showed good reproducibility for the quantification of five analytes in fermentation liquor of Astragalus residue with intra-and inter-day variations less than 3.843%.
机译：中药残留物中含有丰富的纤维素和半纤维素，它们是乙醇生产的潜在可再生碳源。本研究的目的是开发一种快速可靠的方法来评估中药残留物中纤维素和半纤维素的利用率。在本研究中，使用集成脉冲安培检测器（IPAD）通过离子色谱法同时定量了黄芪残渣发酵液中纤维素和半纤维素的关键水解产物（阿拉伯糖，半乳糖，葡萄糖，木糖和纤维二糖）。 HPLC分析在配备GP50梯度泵和ED50 IPAD的Dionex ICS-2500上进行。工作电极和参比电极分别是金电极和Ag / AgCl电极。分离是通过序列号完成的。 002627 Dionex分析柱（2×250 mm）。使用250 mM的氢氧化钠和水作为流动相，流速为0.2 ml / min。柱的温度保持在30℃。验证了该方法的准确性和准确性。在测试范围内，回归方程显示出良好的线性关系（R 2 = 0.9959–0.9984）。五个标准分析物（阿拉伯糖，半乳糖，葡萄糖，木糖和纤维二糖）的检出限和定量限分别为0.067-0.091和0.08-0.23 mg / l。该方法对黄芪残渣发酵液中五种分析物的定量分析具有良好的重现性，日内和日间变化小于3.843％。

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