• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>other >The Involvement of Hemocyte Prophenoloxidase in the Shell-Hardening Process of the Blue Crab Callinectes sapidus
【2h】

The Involvement of Hemocyte Prophenoloxidase in the Shell-Hardening Process of the Blue Crab Callinectes sapidus

机译：血蟹原酚氧化酶参与青蟹壳in的壳强化过程

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cuticular structures of arthropods undergo dramatic molt-related changes from being soft to becoming hard. The shell-hardening process of decapod crustaceans includes sclerotization and mineralization. Hemocyte PPO plays a central role in melanization and sclerotization particularly in wound healing in crustaceans. However, little is known about its role in the crustacean initial shell-hardening process. The earlier findings of the aggregation of heavily granulated hemocytes beneath the hypodermis during ecdysis imply that the hemocytes may be involved in the shell-hardening process. In order to determine if hemocytes and hemocyte PPO have a role in the shell-hardening of crustaceans, a knockdown study using specific CasPPO-hemo-dsRNA was carried out with juvenile blue crabs, Callinectes sapidus. Multiple injections of CasPPO-hemo-dsRNA reduce specifically the levels of CasPPO-hemo expression by 57% and PO activity by 54% in hemocyte lysate at the postmolt, while they have no effect on the total hemocyte numbers. Immunocytochemistry and flow cytometry analysis using a specific antiserum generated against CasPPO show granulocytes, semigranulocytes and hyaline cells as the cellular sources for PPO at the postmolt. Interestingly, the type of hemocytes, as the cellular sources of PPO, varies by molt stage. The granulocytes always contain PPO throughout the molt cycle. However, semigranulocytes and hyaline cells become CasPPO immune-positive only at early premolt and postmolt, indicating that PPO expression in these cells may be involved in the shell-hardening process of C. sapidus.
机译：节肢动物的表皮结构经历了与蜕皮有关的剧烈变化，从软变硬。十足纲甲壳动物的壳硬化过程包括硬化和矿化。血细胞PPO在黑色素和硬化过程中起着核心作用，尤其是在甲壳类动物的伤口愈合中。然而，关于其在甲壳动物最初的壳硬化过程中的作用知之甚少。蜕皮过程中皮下下方大量颗粒状血细胞聚集的早期发现表明，血细胞可能参与了壳硬化过程。为了确定血细胞和血细胞PPO是否在甲壳类动物的壳硬化中起作用，对幼体蓝蟹Callinectes sapidus进行了使用特定CasPPO-hemo-dsRNA的敲低研究。多次注射CasPPO-hemo-dsRNA可以使后蜕皮中的血细胞裂解物中的CasPPO-hemo表达水平降低57％，PO活性降低54％，而对总血细胞数没有影响。使用针对CasPPO产生的特定抗血清的免疫细胞化学和流式细胞术分析显示，粒细胞，半粒细胞和透明细胞是蜕皮后PPO的细胞来源。有趣的是，作为PPO的细胞来源，血细胞的类型随蜕皮期的不同而变化。在整个蜕皮周期中，粒细胞始终含有PPO。但是，半粒细胞和透明细胞仅在蜕皮前和蜕皮后才变为CasPPO免疫阳性，这表明这些细胞中的PPO表达可能参与了镰刀菌的壳硬化过程。

著录项

  • 期刊名称 other
  • 作者

    Javier V. Alvarez; J. Sook Chung;

  • 作者单位
  • 年(卷),期 -1(10),9
  • 年度 -1
  • 页码 e0136916
  • 总页数 20
  • 原文格式 PDF
  • 正文语种
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号