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Optimization of Processing Parameters for Extraction of Amylase Enzyme from Dragon (Hylocereus polyrhizus) Peel Using Response Surface Methodology

机译:响应面法优化龙皮淀粉酶提取工艺参数的优化

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摘要

The main goal of this study was to investigate the effect of extraction conditions on the enzymatic properties of thermoacidic amylase enzyme derived from dragon peel. The studied extraction variables were the buffer-to-sample (B/S) ratio (1 : 2 to 1 : 6, w/w), temperature (−18°C to 25°), mixing time (60 to 180 seconds), and the pH of the buffer (2.0 to 8.0). The results indicate that the enzyme extraction conditions exhibited the least significant (P < 0.05) effect on temperature stability. Conversely, the extraction conditions had the most significant (P < 0.05) effect on the specific activity and pH stability. The results also reveal that the main effect of the B/S ratio, followed by its interaction with the pH of the buffer, was significant (P < 0.05) among most of the response variables studied. The optimum extraction condition caused the amylase to achieve high enzyme activity (648.4 U), specific activity (14.2 U/mg), temperature stability (88.4%), pH stability (85.2%), surfactant agent stability (87.2%), and storage stability (90.3%).
机译:这项研究的主要目的是研究提取条件对龙皮衍生的热酸性淀粉酶的酶学性质的影响。研究的提取变量是缓冲液与样品(B / S)的比率(1:2至1:6,w / w),温度(−18°C至25°),混合时间(60至180秒) ,以及缓冲液的pH值(2.0到8.0)。结果表明,酶提取条件对温度稳定性的影响最小(P <0.05)。相反,萃取条件对比活和pH稳定性的影响最大(P <0.05)。结果还表明,在大多数研究的响应变量中,B / S比的主要影响以及随后与缓冲液pH的相互作用是显着的(P <0.05)。最佳的提取条件使淀粉酶具有较高的酶活性(648.4 U),比活性(14.2 U / mg),温度稳定性(88.4%),pH稳定性(85.2%),表面活性剂稳定性(87.2%)和储存稳定性(90.3%)。

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