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Structure of the PLP Degradative Enzyme 2-Methyl-3-hydroxypyridine-5-carboxylic acid Oxygenase from Mesorhizobium loti MAFF303099 and its Mechanistic Implications

机译：所述pLp降解酶2-甲基-3-羟基吡啶-5-羧酸从慢生根瘤菌根瘤菌maFF303099氧酶和它的机理启示结构

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A vitamin B6 degradative pathway has recently been identified and characterized in Mesorhizobium loti MAFF303099. One of the enzymes on this pathway, 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO), is a flavin-dependent enzyme and catalyzes the oxidative ring opening of 2-methyl-3-hydroxypyridine-5-carboxylic acid to form E-2-acetaminomethylene succinate. The gene for this enzyme has been cloned and the corresponding protein has been overexpressed in Escherichia coli and purified. The crystal structure of MHPCO has been solved to 2.1 Å using SAD phasing with and without the substrate MHPC bound. These crystal structures provide insight into the reaction mechanism and suggest roles for active site residues in the catalysis of a novel oxidative ring-opening reaction.

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Kathryn M. McCulloch; Tathagata Mukherjee; Tadhg P. Begley; Steven E. Ealick;
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年(卷),期 -1(48),19
年度 -1
页码 4139–4149
总页数 23
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1. Structure of the PLP Degradative Enzyme 2-Methyl-3-hydroxypyridine-5-carboxylic Acid Oxygenase from Mesorhizobium loti MAFF303099 and Its Mechanistic Implications [J] . Kathryn M. McCulloch Tathagata Mukherjee Tadhg P. Begley and Steven E. Ealick Biochemistry . 2009,第19期

机译：地上根瘤菌MAFF303099的PLP降解酶2-甲基-3-羟基吡啶-5-羧酸加氧酶的结构及其机理研究
2. Gene Identification and Characterization of the Pyridoxine Degradative Enzyme 4-Pyridoxic Acid Dehydrogenase from the Nitrogen-fixing Symbiotic Bacterium Mesorhizobium loti MAFF303099† [J] . Fei Ge1 Nana Yokochi1 Yu Yoshikane1 Kouhei Ohnishi2 and Toshiharu Yagi1* Journal of Biochemistry . 2008,第5期

机译：固氮共生细菌中生根瘤菌MAFF303099中的吡ox醇降解酶4-吡ox酸脱氢酶的基因鉴定与表征†
3. Gene Identification and Characterization of the Pyridoxine Degradative Enzyme α-( N -Acetylaminomethylene)succinic Acid Amidohydrolase from Mesorhizobium loti MAFF303099 [J] . Baiqiang YUAN, Nana YOKOCHI, Yu YOSHIKANE, Journal of Nutritional Science and Vitaminology . 2008,第3期

机译：地衣芽孢杆菌MAFF303099中吡rid醇降解酶α-（N-乙酰氨基亚甲基）琥珀酸酰胺水解酶的基因鉴定与表征
4. Complete genome structure of Mesorhizobium loti strain MAFF303099 [C] . T. Kaneko, Y. Nakamura, S. Sato, International Congress on Nitrogen Fixation . 2002

机译：Mesorhizobium Loti菌株Maff303099的完整基因组结构
5. Determinants of regioselectivity and electron transfer in the process of heme degradation carried out by the enzyme heme oxygenase in Pseudomonas aeruginosa. [D] . Morgan, Bailey. 2006

机译：铜绿假单胞菌中血红素氧化酶对血红素降解过程中区域选择性和电子转移的决定因素。
6. Gene identification and structural characterization of the PLP degradative protein 3-hydroxy-2-methylpyridine-45-dicarboxylate decarboxylase from Mesorhizobium loti MAFF303099 [O] . Tathagata Mukherjee, Kathryn M. McCulloch, Steven E. Ealick, -1

机译：破伤性中生根瘤菌MAFF303099的PLP降解蛋白3-羟基-2-甲基吡啶-45-二羧酸酯脱羧酶的基因鉴定和结构表征
7. Gene Identification and Characterization of the Pyridoxine Degradative Enzyme .ALPHA.-(N-Acetylaminomethylene)succinic Acid Amidohydrolase from Mesorhizobium loti MAFF303099 [O] . Baiqiang YUAN, Nana YOKOCHI, Yu YOSHIKANE, 2008

机译：基因鉴定与吡哆醇降解酶。α-（n-乙酰氨基甲基）琥珀酸氨基酸氨基氨酸碱酸碱酸碱酸碱酸碱酸碱酸碱酶

Structure of the PLP Degradative Enzyme 2-Methyl-3-hydroxypyridine-5-carboxylic acid Oxygenase from Mesorhizobium loti MAFF303099 and its Mechanistic Implications

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