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首页> 美国卫生研究院文献>Antioxidants >Ultrasound-Assisted Extraction of Total Flavonoids from Pteris cretica L.: Process Optimization HPLC Analysis and Evaluation of Antioxidant Activity
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Ultrasound-Assisted Extraction of Total Flavonoids from Pteris cretica L.: Process Optimization HPLC Analysis and Evaluation of Antioxidant Activity

机译：超声波辅助提取凤尾蕨总黄酮的工艺优化HPLC分析及抗氧化活性的评价

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摘要

In the present work, the ultrasonic-assisted extraction (UAE) of total flavonoids (TF) from L. was optimized by response surface methodology (RSM) on the basis of a single-factor experiment. The optimized UAE parameters were as follows: Ethanol concentration 56.74%, extraction time 45.94 min, extraction temperature 74.27 °C, and liquid/solid ratio 33.69 mL/g. Under the optimized conditions, the total flavonoids yield (TFY) was 4.71 ± 0.04%, which was higher than that obtained by heat reflux extraction (HRE). The extracts were further analyzed by HPLC, and five major flavonoids, including rutin, quercitrin, luteolin, apigenin, and luteolin-7- -glucoside, were identified and quantified. Furthermore, the results of the antioxidant test showed that the TF extract obtained under optimized UAE conditions exhibited good 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical (ABTS •), nitric oxide radical (NO•) scavenging activities, and ferrous ion (Fe ) chelating capacity, with IC values of 74.49, 82.92, 89.12, and 713.41 µg/mL, respectively. Results indicated that the UAE technique developed in this work was an efficient, rapid, and simple approach for the extraction of flavonoids with antioxidant activity from .
机译：在目前的工作中，在单因素实验的基础上，通过响应面法（RSM）对紫苏中总黄酮（TF）的超声辅助提取（UAE）进行了优化。优化的阿联酋参数如下：乙醇浓度56.74％，萃取时间45.94分钟，萃取温度74.27°C，液/固比33.69 mL / g。在最佳条件下，总黄酮收率（TFY）为4.71±0.04％，高于热回流萃取（HRE）。通过HPLC进一步分析提取物，鉴定并定量了五种主要类黄酮，包括芦丁，槲皮苷，木犀草素，芹菜素和木犀草素-7-葡糖苷。此外，抗氧化剂测试的结果表明，在优化的阿联酋条件下获得的TF提取物具有良好的2,2-二苯基-1-吡啶并肼基（DPPH•）和2,2-叠氮基-双（3-乙基苯并噻唑啉-6-磺酸）酸）自由基（ABTS•），一氧化氮自由基（NO•）清除活性和亚铁离子（Fe）螯合能力，IC值分别为74.49、82.92、89.12和713.41 µg / mL。结果表明，这项工作开发的阿联酋技术是一种高效，快速，简单的方法，可从中提取具有抗氧化活性的类黄酮。

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