首页> 美国卫生研究院文献>Cell Division >Toll signaling promotes JNK-dependent apoptosis in Drosophila
【2h】

Toll signaling promotes JNK-dependent apoptosis in Drosophila

机译:收费信号促进果蝇中依赖JNK的细胞凋亡

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Activated Toll signaling triggers cell death in wing development. Light micrographs of adult wings ( – ) and fluorescence micrographs of third instar larval wing discs ( – , – ) are shown. Compared with the -Gal4 controls ( – ), > Toll induces a loss-of-ACV phenotype in adult wings ( ), massive cell death ( ) and apoptosis ( ) in third instar wing discs, all of which are suppressed by expressing two independent RNAi ( – , – ) or Dronc ( – ), but not RNAi ( – ). Expression of Dorsal or depletion of also results in the loss-of-ACV phenotype in adult wings ( , ), and increased apoptotic cell death in third instar wing discs ( , , , ). The lower panels show high magnification view of the boxed areas in upper panels ( – ). For all wings, anterior is to the left and distal up. – Statistical analysis of the ACV phenotype in adult wings ( , n = 50 for each genotype), AO positive cell number ( , n = 9) and cleaved caspase-3 (CC-3) activity ( , n = 10) in wing discs are shown. Error bar indicates standard deviation. One-way ANOVA test was used to compute -values, **** P P ptc>Dorsal flies were reared at 20 °C to avoid lethality at 25 °C, while > - were reared at 29 °C to enhance the expression of RNAi. See Additional file for detailed genotypes. Scale bar: 100 μm
机译:激活的Toll信号触发机翼发育中的细胞死亡。显示了成年翅膀的光显微照片(–)和第三龄幼虫的翅膀圆盘(–,–)的荧光显微照片。与-Gal4对照(-)相比,> Toll会在成年双翅中诱导ACV丧失表型(),在三龄翼翅中引起大量细胞死亡()和凋亡(),所有这些都通过表达两个独立的个体而被抑制RNAi(–,–)或Dronc(–),但不是RNAi(–)。背鳍的表达或耗竭也会导致成年翅中ACV表型的丧失(),并导致第三龄翅翅中凋亡细胞死亡的增加(,,)。下面板以高放大倍数显示了上面板(–)中的方框区域。对于所有的机翼,前部位于左侧,远端向上。 –成年翅中ACV表型的统计分析(每种基因型,n = 50),AO阳性细胞数(n == 9)和裂解的半胱氨酸天冬氨酸蛋白酶3(CC-3)活性(n-3 = 10)显示。误差线表示标准偏差。单向方差分析用于计算-值,**** P P ptc>背蝇在20°C饲养以避免在25°C致死,而>-在29°C饲养以增强其表达。 RNAi。有关详细的基因型,请参见其他文件。比例尺:100μm

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号