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Decolorization and Detoxification of Synthetic Dyes by Mexican Strains of Trametes sp.

机译:墨西哥菌株Trametes sp。对合成染料的脱色和脱毒。

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摘要

Laccases have attracted a great deal of interest because of their remarkable ability for the degradation of synthetic dyes present in wastewaters. New laccase producing sources with robust operational and functional properties are being continuously explored. In this work, the potential for the decolorization and detoxification of synthetic dyes was evaluated in two Mexican strains of the genus . The decolorization capacity of LE130 and sp. LA1 was tested in solid and liquid media. The phytotoxicity of the degradation products was determined using and seeds. In solid media, both strains showed a higher decolorization capacity ( ≤ 0.05) than ATCC 24725, which is known to be very efficient in lignin and dye-degradation. They produced laccase as the main ligninolytic enzyme; LE130 secreted a single isoform of 43.9 kDa, while sp. LA1 produced three isoforms of 67.3, 58.6 and 52.7 kDa, respectively. sp. LA1 culture fluids were capable of decolorizing and detoxifying chemically diverse dyes (anthraquinonic dye Remazol Brilliant Blue R, azoic Reactive Black 5 and triphenylmethane Crystal Violet) without the addition of redox mediators. Therefore, this could be considered as a new laccase source which could be potentially competitive in the bioremediation of dye-containing wastewaters.
机译:漆酶由于其降解废水中合成染料的卓越能力而引起了极大的兴趣。具有健壮的操作和功能特性的新漆酶生产来源正在不断探索中。在这项工作中,在两个墨西哥属中评估了合成染料的脱色和脱毒潜力。 LE130和sp的脱色能力。 LA1在固体和液体介质中进行了测试。使用和种子确定降解产物的植物毒性。在固体培养基中,两种菌株均比ATCC 24725表现出更高的脱色能力(≤0.05),这在木质素和染料降解方面非常有效。他们产生了漆酶作为主要的木质素分解酶。 LE130分泌一个43.9 kDa的同工型,而sp。 LA1分别产生了67.3、58.6和52.7 kDa的三种同工型。 sp。 LA1培养液能够在不添加氧化还原介体的情况下对多种化学染料(蒽醌染料Remazol艳蓝R,偶氮活性黑5和三苯甲烷结晶紫)进行脱色和脱毒。因此,这可以被认为是一种新的漆酶源,在含染料废水的生物修复中具有潜在的竞争力。

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