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首页> 美国卫生研究院文献>Antioxidants >Enrichment in Antioxidant Flavonoids of Stamen Extracts from Nymphaea lotus L. Using Ultrasonic-Assisted Extraction and Macroporous Resin Adsorption
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Enrichment in Antioxidant Flavonoids of Stamen Extracts from Nymphaea lotus L. Using Ultrasonic-Assisted Extraction and Macroporous Resin Adsorption

机译：使用超声波辅助提取和大孔树脂吸附的脉络提取物的抗氧化剂黄酮类化合物中的富集

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L. is the medicinal plant that has long been used for food, cosmetics and traditional medicines in Africa and Asia since ancient times. Its flavonoids and other interesting phytochemical compounds from rhizome, leaf and the whole flowers have been reported in the previous published research. However, stamens, which are essential for reproductive functions, may also represent new alternative sources of potential antioxidant flavonoids, as investigated in this study. The innovative green chemistry methods, i.e., ultrasound-assisted extraction (UAE) as well as a macroporous resin (MPR) purification procedure, were employed in this current research. Using a full factorial design coupled to three-dimensional (3D) surface plot methodology, the influence of three variables, namely aqEtOH concentration (ranging from 50 to 100% ( / ), US frequency (ranging from 0 (no US applied) to 45 kHz), and the extraction duration (ranging from 20 to 60 min), were evaluated. Five MPRs with different surface areas, average pore diameters, matrix types and polarities were also investigated for the purification of total flavonoids. The optimal UAE condition is 90% ( / ) aqEtOH with 34.65 khz ultrasonic frequency and 46 min of extraction duration. Compared with the conventional heat reflux extraction (HRE) method, a significant 1.35-fold increase in total flavonoids content was obtained using optimized UAE conditions (169.64 for HRE vs. 235.45 mg/g dry weight for UAE), causing a 2.80-fold increase when this UAE associated with MPR purification (475.42 mg/g dry weight). In vitro cell free antioxidant activity of stamen extracts and in cellulo antioxidant investigation using yeast model showed the same trend, indicating that the best antioxidant flavonoid can be found in UAE coupled with MPR purification. Moreover, in the yeast model, the expression of key antioxidant genes such as and were expressed at the highest level in yeast cells treated with the extract from UAE together with MPR purification. Consequently, it can be seen that the UAE combined with MPR purification can help enhance the flavonoid antioxidant potential of the stamens extract from this medicinal species.
机译：L.自古以来一直用于非洲和亚洲的食品，化妆品和传统药物的药用植物。目前已发表的研究报告了其来自根茎，叶和整个花的异味和其他有趣的植物化合物。然而，对于生殖功能至关重要的雄蕊也可以代表潜在抗氧化类黄酮类的新替代来源，如本研究所研究。在本前研究中，采用了创新的绿色化学方法，即超声辅助提取（UAE）以及大孔树脂（MPR）纯化程序。使用耦合到三维（3D）表面图方法的完整因子设计，三个变量的影响，即AqetOH浓度（范围为50至100％（/），美国频率（从0（没有）到45评估KHz）和提取持续时间（范围从20至60分钟）。还研究了不同表面积，平均孔径，基质类型和极性的五种MPR，用于纯化总黄酮。最佳的阿联酋病症是90 ％（/）具有34.65kHz超声波频率和46分钟的提取持续时间的AQETOH。与常规的热回流萃取（HRE）方法相比，使用优化的UAE条件（169.64 vs，获得总黄酮含量的显着1.35倍的增加。 。当UAE与MPR纯化相关（475.42mg / g干重）时，235.45mg / g的干重，导致2.80倍的增加（475.42mg / g干重）。体外细胞无氧化活性雄蕊提取物和纤维素抗氧化使用酵母模型的蚂蚁调查显示出相同的趋势，表明最佳的抗氧化类黄酮可以在UAE与MPR纯化结合时发现。此外，在酵母模型中，在用来自阿联酋的提取物处理的酵母细胞中的最高水平和MPR纯化处理的关键抗氧化基因如和表达的表达。因此，可以看出，阿联酋与MPR纯化结合可以有助于提高雄蕊从该药物中提取雄蕊的黄酮抗氧化潜力。

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