• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Poultry Science >Research Note: Epidemiological cutoff values and acquired resistance mechanisms of three veterinary antibiotics against Escherichia coli from chicken respiratory tract infections
【2h】

Research Note: Epidemiological cutoff values and acquired resistance mechanisms of three veterinary antibiotics against Escherichia coli from chicken respiratory tract infections

机译：研究注意：流行病学截止值和三种兽医抗生素对来自鸡呼吸道感染大肠杆菌的抗性机制

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Florfenicol, apramycin, and danofloxacin are antibiotics approved only for veterinary use and that have good therapeutic effects on chicken respiratory infections caused by Escherichia coli. We established epidemiological cutoff values (ECV) for these antibiotics using 363 E. coli isolates from tracheal samples of chickens in 5 veterinary clinics in Guangdong Province, China. The minimum inhibitory concentrations (MIC) were determined using the agar dilution method as per Clinical and Laboratory Standards Institution guidelines. The ECV were then calculated using the statistical method and verified by normalized resistance interpretation and ECOFFinder software programs. The ECV of florfenicol, apramycin, and danofloxacin against E. coli were 16, 16, and 0.125 μg/mL, respectively. Susceptibility tests indicated that these isolates were resistant to florfenicol (66.7%), apramycin (22.3%), and danofloxacin (92.3%). Strains carrying floR were distributed in the range of MIC ≥32 μg/mL for florfenicol. Apramycin resistance was found in 77 strains (77/363, 21.1%), and isolates that carried aac(3)-IV were all in the range of MIC ≥512 μg/mL. Danofloxacin resistance was found in the range of MIC ≤0.125 μg/mL, but there were no mutations in the quinolone resistance–determining regions and plasmid-mediated quinolone resistance genes qnrA, qnrB, qnrC, qnrD, aac-(6′)-Ib-cr, qep, and oqxB. The presence of the qnrS gene was verified in a few of the strains with an MIC of 0.06 μg/mL. The establishment of ECV was significant for monitoring of resistance development and therapy guidance.
机译：Florfenicol，Apramycin和Danofloxacin是抗生素，只批准兽医用途，对大肠杆菌引起的鸡呼吸道感染具有良好的治疗效果。我们使用来自中国广东省5兽医诊所的鸡气管样本的363大肠杆菌分离物建立了这些抗生素的流行病学截止值（ECV）。根据临床和实验室标准制定指南，使用琼脂稀释法测定最小抑制浓度（MIC）。然后使用统计方法计算ECV并通过归一化电阻解释和ECOffinder软件程序验证。 Florfenicol，Apramycin和Danofloxacin的ECV分别对大肠杆菌进行16,16和0.125μg/ ml。易感性试验表明，这些分离株对弗洛林尼（66.7％），尿嘧啶（22.3％）和丹木霉素（92.3％）耐药。携带Flor的菌株分布在氟芬酚的MIC≥32μg/ ml的范围内。在77株（77/363,21.1％）中发现了477株（77/363,21.1％），并且携带AAC（3）-IV的分离物全部在MIC≥512μg/ ml的范围内。在MIC≤0.125μg/ mL的范围内发现丹氟氯嘧啶抗性，但喹耳抗性测定区和质粒介导的喹啉抗性基因QNRA，QNRB，QNRC，QNRD，AAC-（6'） - IB中没有突变-CR，QEP和OQXB。在少量菌株中验证QNR基因的存在，MIC为0.06μg/ ml。 ECV的建立对于监测抗性发展和治疗指导是显着的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号