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首页> 美国卫生研究院文献>Frontiers in Microbiology >High-grain feeding causes strong shifts in ruminal epithelial bacterial community and expression of Toll-like receptor genes in goats
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High-grain feeding causes strong shifts in ruminal epithelial bacterial community and expression of Toll-like receptor genes in goats

机译：高粮喂养导致山羊瘤胃上皮细菌群落的强烈变化和Toll样受体基因的表达

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High-grain (HG) feeding used in intensive goat production can affect the physiology of the rumen wall, but the changes induced in the epimural bacterial community and host Toll-like receptors (TLRs) are not well understood. In this study, 10 male goats were randomly allocated to two groups and fed either a hay diet (0% grain; n = 5) or an HG diet (65% grain; n = 5). The changes in the ruminal epithelial bacterial community and expression of TLRs during long-term (7 weeks) HG feeding were determined using pyrosequencing and quantitative real-time polymerase chain reaction. Principal coordinate analysis and analysis of molecular variance (AMOVA) results showed that HG feeding caused a strong shift in bacterial composition and structure. At the genus level, our data revealed that it increased the relative abundance of taxa Butyrivibrio, unclassified Clostridiales, Mogibacterium, unclassified Anaerolineaceae, and Succiniclasticum, and decreased the proportion of unclassified Ruminococcaceae, unclassified Rikenellaceae, unclassified Erysipelotrichaceae, Howardella, and unclassified Neisseriaceae. The HG-fed goats also exhibited upregulation of the relative mRNA expression of TLR2, TLR3, and TLR5 in the rumen epithelium (P < 0.05). Correlation analysis revealed that the increase in TLR expression was associated with changes in the relative abundance of ruminal epithelial bacteria. This study provides a first insight into the adaptive response of ruminal epithelial bacterial populations to HG feeding in goats and shows that these changes were associated with alterations in TLR expression. These findings provide new insight into understanding of host–microbial relationships in ruminants.
机译：在山羊集约化生产中使用高谷物（HG）喂养会影响瘤胃壁的生理，但是在硬膜外细菌群落和宿主Toll样受体（TLR）中引起的变化尚不十分清楚。在这项研究中，将10只公山羊随机分为两组，分别饲喂干草（0％谷物； n = 5）或HG饲料（65％谷物； n = 5）。使用焦磷酸测序和定量实时聚合酶链反应确定长期（7周）HG喂养期间瘤胃上皮细菌群落的变化和TLR的表达。主坐标分析和分子变异分析（AMOVA）结果表明，HG进食引起细菌组成和结构的强烈变化。在属水平上，我们的数据显示它增加了分类单元Butyrivibrio，未分类的梭状芽孢杆菌，Mogi细菌，未分类的厌氧菌科和琥珀色弹菌菌的相对丰度，并降低了未分类的鲁美球藻科，未分类的里克氏菌科，未分类的刺毛菊科，霍乱氏菌，霍华氏菌的比例。用HG饲​​喂的山羊还显示瘤胃上皮中TLR2，TLR3和TLR5的相对mRNA表达上调（P <0.05）。相关分析表明，TLR表达的增加与瘤胃上皮细菌相对丰度的变化有关。这项研究提供了对山羊瘤胃上皮细菌种群对HG摄食的适应性反应的初步见解，并表明这些变化与TLR表达的改变有关。这些发现为反刍动物对宿主-微生物关系的理解提供了新的见解。

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