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Root Associated Bacillus sp. Improves Growth, Yield and Zinc Translocation for Basmati Rice (Oryza sativa) Varieties

机译:根相关芽孢杆菌改善印度香米(Oryza sativa)品种的生长,产量和锌转运

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摘要

Plant associated rhizobacteria prevailing in different agro-ecosystems exhibit multiple traits which could be utilized in various aspect of sustainable agriculture. Two hundred thirty four isolates were obtained from the roots of basmati-385 and basmati super rice varieties growing in clay loam and saline soil at different locations of Punjab (Pakistan). Out of 234 isolates, 27 were able to solubilize zinc (Zn) from different Zn ores like zinc phosphate [Zn3 (PO4)2], zinc carbonate (ZnCO3) and zinc oxide (ZnO). The strain SH-10 with maximum Zn solubilization zone of 24 mm on Zn3 (PO4)2ore and strain SH-17 with maximum Zn solubilization zone of 14–15 mm on ZnO and ZnCO3ores were selected for further studies. These two strains solubilized phosphorous (P) and potassium (K) in vitro with a solubilization zone of 38–46 mm and 47–55 mm respectively. The strains also suppressed economically important rice pathogens Pyricularia oryzae and Fusarium moniliforme by 22–29% and produced various biocontrol determinants in vitro. The strains enhanced Zn translocation toward grains and increased yield of basmati-385 and super basmati rice varieties by 22–49% and 18–47% respectively. The Zn solubilizing strains were identified as Bacillus sp. and Bacillus cereus by 16S rRNA gene analysis.
机译:在不同农业生态系统中普遍存在的与植物相关的根际细菌表现出多种特征,可用于可持续农业的各个方面。从在旁遮普邦(巴基斯坦)不同地区的黏土壤和盐渍土中生长的basmati-385和basmati超级稻品种的根中获得了234个分离株。在234种分离物中,有27种能够溶解来自不同锌矿石(如磷酸锌[Zn3(PO4)2],碳酸锌(ZnCO3)和氧化锌(ZnO))的锌(Zn)。选择在Zn3(PO4)2ore上最大Zn溶解区为24 mm的SH-10菌株和在ZnO和ZnCO3ores上最大Zn溶解区为14-15 mm的SH-17菌株进行进一步研究。这两个菌株在体外分别溶解了38-46 mm和47-55 mm的磷(P)和钾(K)。该菌株还抑制了重要的水稻病原体稻瘟病菌和稻镰刀菌22-29%,并在体外产生了多种生物控制决定因素。该菌株增强了锌向籽粒的转运,使basmati-385和超级basmati水稻品种的产量分别增加了22–49%和18–47%。锌增溶菌株被鉴定为芽孢杆菌。和蜡样芽孢杆菌通过16S rRNA基因分析。

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