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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Simple Sequence Repeat (SSR) Genetic Linkage Map of D Genome Diploid Cotton Derived from an Interspecific Cross between Gossypium davidsonii and Gossypium klotzschianum
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Simple Sequence Repeat (SSR) Genetic Linkage Map of D Genome Diploid Cotton Derived from an Interspecific Cross between Gossypium davidsonii and Gossypium klotzschianum

机译：D基因组二倍体棉花的简单序列重复（SSR）遗传连锁图谱来源于棉花（Gossypium davidsonii）和克氏棉（Gossypium klotzschianum）之间的种间杂交

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The challenge in tetraploid cotton cultivars is the narrow genetic base and therefore, the bottleneck is how to obtain interspecific hybrids and introduce the germplasm directly from wild cotton to elite cultivars. Construction of genetic maps has provided insight into understanding the genome structure, interrelationships between organisms in relation to evolution, and discovery of genes that carry important agronomic traits in plants. In this study, we generated an interspecific hybrid between two wild diploid cottons, Gossypium davidsonii and Gossypium klotzschianum, and genotyped 188 F2:3 populations in order to develop a genetic map. We screened 12,560 SWU Simple Sequence Repeat (SSR) primers and obtained 1000 polymorphic markers which accounted for only 8%. A total of 928 polymorphic primers were successfully scored and only 728 were effectively linked across the 13 chromosomes, but with an asymmetrical distribution. The map length was 1480.23 cM, with an average length of 2.182 cM between adjacent markers. A high percentage of the markers on the map developed, and for the physical map of G. raimondii, exhibited highly significant collinearity, with two types of duplication. High level of segregation distortion was observed. A total of 27 key genes were identified with diverse roles in plant hormone signaling, development, and defense reactions. The achievement of developing the F2:3 population and its genetic map constructions may be a landmark in establishing a new tool for the genetic improvement of cultivars from wild plants in cotton. Our map had an increased recombination length compared to other maps developed from other D genome cotton species.
机译：四倍体棉花品种面临的挑战是狭窄的遗传基础，因此，瓶颈在于如何获得种间杂种并将种质直接从野生棉引入优良品种。遗传图谱的构建为了解基因组结构，与进化有关的生物之间的相互关系以及发现具有植物重要农艺性状的基因提供了见识。在这项研究中，我们生成了两个野生二倍体棉，棉和棉之间的种间杂种，并对188 F2：3种群进行了基因分型，以建立遗传图谱。我们筛选了12,560个SWU简单序列重复（SSR）引物，获得了1000个多态性标记，仅占8％。总共成功地对928个多态性引物进行了评分，只有728个有效地连接了13条染色体，但分布不对称。地图长度为1480.23 cM，相邻标记之间的平均长度为2.182 cM。在地图上，以及雷蒙德氏乳杆菌的物理地图上，很大比例的标记物表现出高度显着的共线性，具有两种重复类型。观察到高水平的偏析变形。总共鉴定出27个关键基因，它们在植物激素信号传导，发育和防御反应中具有多种作用。 F2：3种群的发展及其遗传图谱的构建，可能是建立新的遗传改良棉花野生植物品种遗传新工具的里程碑。与从其他D基因组棉种开发的其他图谱相比，我们的图谱的重组长度增加了。

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