• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>PLoS Clinical Trials >Composition and variation of respiratory microbiota in healthy military personnel
【2h】

Composition and variation of respiratory microbiota in healthy military personnel

机译：健康军人呼吸菌群的组成和变化

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Certain occupational and geographical exposures have been associated with an increased risk of lung disease. As a baseline for future studies, we sought to characterize the upper respiratory microbiomes of healthy military personnel in a garrison environment. Nasal, oropharyngeal, and nasopharyngeal swabs were collected from 50 healthy active duty volunteers eight times over the course of one year (1107 swabs, completion rate = 92.25%) and subjected to pyrosequencing of the V1–V3 region of 16S rDNA. Respiratory bacterial taxa were characterized at the genus level, using QIIME 1.8 and the Ribosomal Database Project classifier. High levels of Staphylococcus, Corynebacterium, and Propionibacterium were observed among both nasal and nasopharyngeal microbiota, comprising more than 75% of all operational taxonomical units (OTUs). In contrast, Streptococcus was the sole dominant bacterial genus (approximately 50% of all OTUs) in the oropharynx. The average bacterial diversity was greater in the oropharynx than in the nasal or nasopharyngeal region at all time points. Diversity analysis indicated a significant overlap between nasal and nasopharyngeal samples, whereas oropharyngeal samples formed a cluster distinct from these two regions. The study produced a large set of pyrosequencing data on the V1–V3 region of bacterial 16S rDNA for the respiratory microbiomes of healthy active duty Service Members. Pre-processing of sequencing reads showed good data quality. The derived microbiome profiles were consistent both internally and with previous reports, suggesting their utility for further analyses and association studies based on sequence and demographic data.
机译：某些职业和地理暴露与肺病风险增加相关。作为未来研究的基线，我们试图表征驻军环境中健康军事人员的上呼吸道微生物组。在一年的过程中，从50位健康的现役志愿者中收集了8次鼻，口咽和鼻咽拭子（1107拭子，完成率= 92.25％），并对16S rDNA的V1-V3区进行焦磷酸测序。使用QIIME 1.8和Ribosomal Database Project分类器在属水平上鉴定呼吸细菌类群。在鼻和鼻咽微生物群中均观察到高水平的葡萄球菌，棒状杆菌和丙酸杆菌，占所有操作分类单位（OTU）的75％以上。相反，链球菌是口咽中唯一的优势细菌属（约占所有OTU的50％）。在所有时间点，口咽中的平均细菌多样性均大于鼻或鼻咽区域。多样性分析表明鼻和鼻咽样品之间存在明显的重叠，而口咽样品形成了与这两个区域不同的簇。该研究针对健康现役军人的呼吸微生物群，在细菌16S rDNA的V1-V3区产生了大量的焦磷酸测序数据。测序读段的预处理显示出良好的数据质量。得出的微生物组图谱在内部和以前的报告中均一致，表明它们可用于基于序列和人口统计学数据的进一步分析和关联研究。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号