• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Wiley-Blackwell Online Open >Culturable prokaryotic diversity of deep gas hydrate sediments: first use of a continuous high-pressure anaerobic enrichment and isolation system for subseafloor sediments (DeepIsoBUG)
【2h】

Culturable prokaryotic diversity of deep gas hydrate sediments: first use of a continuous high-pressure anaerobic enrichment and isolation system for subseafloor sediments (DeepIsoBUG)

机译：深层天然气水合物沉积物的可培养原核生物多样性：首次对海底沉积物使用连续高压厌氧富集和隔离系统（DeepIsoBUG）

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Deep subseafloor sediments may contain depressurization-sensitive, anaerobic, piezophilic prokaryotes. To test this we developed the DeepIsoBUG system, which when coupled with the HYACINTH pressure-retaining drilling and core storage system and the PRESS core cutting and processing system, enables deep sediments to be handled without depressurization (up to 25 MPa) and anaerobic prokaryotic enrichments and isolation to be conducted up to 100 MPa. Here, we describe the system and its first use with subsurface gas hydrate sediments from the Indian Continental Shelf, Cascadia Margin and Gulf of Mexico. Generally, highest cell concentrations in enrichments occurred close to in situ pressures (14 MPa) in a variety of media, although growth continued up to at least 80 MPa. Predominant sequences in enrichments were Carnobacterium, Clostridium, Marinilactibacillus and Pseudomonas, plus Acetobacterium and Bacteroidetes in Indian samples, largely independent of media and pressures. Related 16S rRNA gene sequences for all of these Bacteria have been detected in deep, subsurface environments, although isolated strains were piezotolerant, being able to grow at atmospheric pressure. Only the Clostridium and Acetobacterium were obligate anaerobes. No Archaea were enriched. It may be that these sediment samples were not deep enough (total depth 1126–1527 m) to obtain obligate piezophiles.
机译：深层海底沉积物可能含有对减压敏感的厌氧，亲压原核生物。为了对此进行测试，我们开发了DeepIsoBUG系统，该系统与HYACINTH保压钻井和岩心存储系统以及PRESS岩心切割和处理系统结合使用，能够处理深层沉积物而无需减压（最高25 MPa）和厌氧原核生物富集并进行高达100 MPa的隔离。在这里，我们描述了该系统及其首次用于印度大陆架，卡斯卡迪亚边界和墨西哥湾的地下天然气水合物沉积物。通常，尽管生长持续至少达到80 MPa，但在各种培养基中富集的最高细胞浓度却接近原位压力（14 MPa）。富集的主要序列是印度样品中的食肉杆菌，梭菌，马里尼乳杆菌和假单胞菌，以及醋杆菌和拟杆菌，其在很大程度上与培养基和压力无关。尽管分离出的菌株具有耐压电性，并且能够在大气压下生长，但已在深层地下环境中检测到了所有这些细菌的相关16S rRNA基因序列。只有梭菌和醋杆菌为专性厌氧菌。没有古细菌丰富。这些沉积物样本可能不够深（总深度为1126-1527 m），无法获得专性的亲压性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号