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首页> 外文期刊>Acta Physiologiae Plantarum >Overexpressing ArabidopsisKNAT1 gene in Celosia plumosus L. causes modification of plant morphology
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Overexpressing ArabidopsisKNAT1 gene in Celosia plumosus L. causes modification of plant morphology

机译：在鸡冠花中过表达拟南芥KNAT1基因导致植物形态的改变

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Celosia plumosus L. is one of the most common gardening plants worldwide. The C. plumosus L. transformation system established by other research team should be useful for modifying physiological, medicinal, and horticultural traits. Though morphological modification of C. plumosus L. would be critical in terms of its commercial value, few attempts have been reported until now. In modification of gardening plants, many potentially useful genes that are involved in the pathways associated with flower and plant morphology have been cloned. Transcription factors regulating plant development and biosynthetic or regulatory genes involved in plant hormones are common candidates. KNAT1, isolated from Arabidopsis, is a knotted1-like homeobox (knox), and it is expressed in the shoot apical meristem and not in determinate organs. Previous reports have indicated that ectopic expression of KNAT1 in Arabidopsis changes simple leaves into lobed leaves or rumpled leaves. Therefore, this transcription factor might regulate plant morphology. In this work, we constructed the binary vector pBIN-pMD-18T, which contained the GFP and KNAT1 coding sequence, and transformed them into C. plumosus L. plants by Agrobacterium tumefaciens. Our results show that the KNAT1-GFP fusion protein was selectively located in the nucleus of roots and leaves, consistent with a potential function as transcription factor reported by other research teams. We observed that some 35S:KNAT1-GFP plants display lobed or rumpled leaves, partite leaves. Moreover, overall plant morphology was altered in extreme phenotype category, i.e., dwarfed. Together, these morphological modifications of C. plumosus L. can have potential practical applications.
机译：Celosia plumosus L.是世界上最常见的园艺植物之一。由其他研究小组建立的C. plumosus L.转化系统应有助于改变生理，医学和园艺特性。尽管就其商业价值而言，C。plumosus L.的形态学修饰至关重要，但迄今为止尚未有任何尝试报道。在园艺植物的修饰中，已经克隆了许多与花和植物形态相关的途径中涉及的潜在有用的基因。调节植物发育的转录因子以及植物激素中涉及的生物合成或调节基因是常见的候选者。从拟南芥中分离出的KNAT1是一个类似knotted1的同源盒（knox），它在茎尖分生组织中表达，而不是在确定的器官中表达。以前的报道表明，KNAT1在拟南芥中的异位表达将单叶变成浅叶或皱叶。因此，该转录因子可能调节植物形态。在这项工作中，我们构建了包含GFP和KNAT1编码序列的二元载体pBIN-pMD-18T，并通过根癌农杆菌将其转化为C. plumosus L.植物。我们的结果表明，KNAT1-GFP融合蛋白选择性地位于根和叶的核中，与其他研究小组报道的潜在转录因子功能一致。我们观察到一些35S：KNAT1-GFP植物显示有浅叶或皱巴巴的叶，部分叶。此外，在极端表型类别，即矮化中，整体植物形态发生了改变。一起，C。plumosus L.的这些形态修饰可能具有潜在的实际应用。

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