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首页> 外文期刊>Advanced Functional Materials >Colloidal Stability and Surface Chemistry Are Key Factors for the Composition of the Protein Corona of Inorganic Gold Nanoparticles
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Colloidal Stability and Surface Chemistry Are Key Factors for the Composition of the Protein Corona of Inorganic Gold Nanoparticles

机译：胶体稳定性和表面化学是无机金纳米颗粒蛋白质电晕组成的关键因素

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摘要

To study the influence of colloidal stability on protein corona formation, gold nanoparticles are synthesized with five distinct surface modifications: coating with citric acid, bis(p-sulfonatophenyl)phenylphosphine dihydrate dipotassium salt, thiol-terminated methoxy-polyethylene glycol, dodecylamine-grafted poly(isobutylene-alt-maleic anhydride), and dodecylamine-grafted poly(isobutylene-alt-maleic anhydride) conjugated with polyethylene glycol. The nanoparticles are incubated with serum or bronchoalveolar lavage fluid from C57BL/6 mice (15 min or 24 h) to assess the effect of differential nanoparticle surface presentation on protein corona formation in the air-blood barrier exposure pathway. Proteomic quantification and nanoparticle size measurements are used to assess protein corona formation. We show that surface modification has a clear effect on the size and the composition of the protein corona that is related to the colloidal stability of the studied nanoparticles. Additionally, differences in the composition and size of the protein corona are shown between biological media and duration of exposure, indicating evolution of the corona through this exposure pathway. Consequently, a major determinant of protein corona formation is the colloidal stability of nanoparticles in biological media and chemical or environmental modification of the nanoparticles alters the surface presentation of the functional epitope in vivo. Therefore, the colloidal stability of nanoparticles has a decisive influence on nano-bio interactions.
机译：为了研究胶体稳定性对蛋白质电晕形成的影响，合成了具有五个不同表面修饰的金纳米颗粒：柠檬酸涂层，双（对-磺基苯基）苯基膦二水合二钾盐，巯基封端的甲氧基-聚乙二醇，十二烷基胺接枝的聚（异丁烯-马来酸酐）和与聚乙二醇共轭的十二烷基接枝的聚（异丁烯-马来酸酐）。将纳米颗粒与C57BL / 6小鼠的血清或支气管肺泡灌洗液温育（15分钟或24小时），以评估不同的纳米颗粒表面表现形式对气血屏障暴露途径中蛋白质电晕形成的影响。蛋白质组学定量和纳米粒度测量用于评估蛋白质电晕的形成。我们表明表面改性对蛋白质电晕的大小和组成具有明显的影响，这与研究的纳米粒子的胶体稳定性有关。另外，在生物介质和暴露时间之间显示出蛋白质电晕的组成和大小的差异，表明电晕通过该暴露途径的进化。因此，蛋白质电晕形成的主要决定因素是纳米粒子在生物介质中的胶体稳定性，并且纳米粒子的化学或环境修饰改变了体内功能性表位的表面表现。因此，纳米颗粒的胶体稳定性对纳米生物相互作用具有决定性的影响。

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  • 来源
    《Advanced Functional Materials》 |2017年第42期|1701956.1-1701956.9|共9页
  • 作者

    Johnston Blair D.; Kreyling Wolfgang G.; Pfeiffer Christian; Schaeffler Martin; Sarioglu Hakan; Ristig Simon; Hirn Stephanie; Haberl Nadine; Thalhammer Stefan; Hauck Stefanie M.; Semmler-Behnke Manuela; Epple Matthias; Huehn Jonas; Del Pino Pablo; Parak Wolfgang J.;

  • 作者单位

    German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Lung Biol & Dis, D-85764 Neuherberg, Germany;

    German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Lung Biol & Dis, D-85764 Neuherberg, Germany|German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Epidemiol 2, D-85764 Neuherberg, Germany;

    Philipps Univ Marburg, Dept Phys, D-35032 Marburg, Germany;

    German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Lung Biol & Dis, D-85764 Neuherberg, Germany;

    German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Res Unit Prot Sci, D-85764 Neuherberg, Germany;

    Univ Duisburg Essen, Inorgan Chem, D-45141 Essen, Germany|Univ Duisburg Essen, Ctr Nanointegrat Duisburg Essen CeNIDE, D-45141 Essen, Germany;

    German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Lung Biol & Dis, D-85764 Neuherberg, Germany;

    German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Lung Biol & Dis, D-85764 Neuherberg, Germany;

    German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Radiat Protect, D-85764 Neuherberg, Germany|Univ Augsburg, Inst Phys, D-86135 Augsburg, Germany;

    German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Res Unit Prot Sci, D-85764 Neuherberg, Germany;

    German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Lung Biol & Dis, D-85764 Neuherberg, Germany|Bavarian Hlth & Food Safety Author, D-85764 Oberschleissheim, Germany;

    Univ Duisburg Essen, Inorgan Chem, D-45141 Essen, Germany|Univ Duisburg Essen, Ctr Nanointegrat Duisburg Essen CeNIDE, D-45141 Essen, Germany;

    Philipps Univ Marburg, Dept Phys, D-35032 Marburg, Germany;

    Philipps Univ Marburg, Dept Phys, D-35032 Marburg, Germany|CIC BiomaGUNE, San Sebastian 20009, Spain|Univ Santiago de Compostela, Ctr Singular Invest Quim Biol & Mat Mol CiQUS, Santiago, Spain|Univ Santiago de Compostela, Dept Fis Particulas, Santiago, Spain;

    Philipps Univ Marburg, Dept Phys, D-35032 Marburg, Germany|CIC BiomaGUNE, San Sebastian 20009, Spain;

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  • 正文语种 eng
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  • 关键词

    colloidal stability; gold nanoparticles; protein coronas; surface chemistry dependence;

    机译：胶体稳定性;金纳米颗粒;蛋白质电晕;表面化学依赖性;

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