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首页> 外文期刊>Applied Microbiology and Biotechnology >Production of glucose–fructose oxidoreductase and ethanol by Zymomonas mobilis ATCC 29191 in medium containing corn steep liquor as a source of vitamins
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Production of glucose–fructose oxidoreductase and ethanol by Zymomonas mobilis ATCC 29191 in medium containing corn steep liquor as a source of vitamins

机译:运动发酵单胞菌ATCC 29191在含有玉米浆作为维生素来源的培养基中生产葡萄糖-果糖氧化还原酶和乙醇

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摘要

Different concentrations of corn steep liquor (CSL) were tested in the cultivation of Zymomonas mobilis. Cell growth, ethanol production, and the formation of glucose–fructose oxidoreductase (GFOR) and glucono-δ-lactonase (GL), the enzymes responsible for the bio-production of gluconic acid and sorbitol, were examined. The cell yields using 25 g CSL l–1 and 40 g CSL l–1 (Y X/S≈0.031 g g–1) were close to that obtained with 5 g yeast extract (YE) l–1. With 5 g CSL l–1 and 15 g CSL l–1, the nutritional limitation led to smaller Y X/S. Using 100 g CSL l–1 produced an inhibitory effect on cell growth. Similar ethanol yields (92–95%) were calculated for each concentration of CSL and also for YE medium. The highest specific GFOR/GL activities (13.2–13.5 U g–1 dry cell) were reached with 25 g CSL l–1 and 40 g CSL l–1, values comparable to that achieved with 5 g YE l–1. The results confirm that CSL is an effective and cheap supplement for Z. mobilis medium, increasing the economic potential of a large-scale bio-production of sorbitol and gluconic acid by untreated Z. mobilis cells. The economic feasibility of the process is discussed.
机译:在运动发酵单胞菌的培养中测试了不同浓度的玉米浆(CSL)。检查了细胞的生长,乙醇的产生以及葡萄糖-果糖氧化还原酶(GFOR)和葡萄糖酸-δ-内酰胺酶(GL)的形成,这些酶负责葡萄糖酸和山梨糖醇的生物生产。使用25 g CSL l-1 和40 g CSL l-1 (YX / S ≈0.031gg-1 )的细胞产量接近于5 g酵母提取物(YE)1-1 。由于营养限制摄入量为5 g CSL l-1 和15 g CSL l-1 ,导致Y X / S 变小。使用100 g CSL 1-1 对细胞生长产生抑制作用。对于每种浓度的CSL以及YE培养基,都计算出了相似的乙醇收率(92–95%)。 25 g CSL l-1 和40 g CSL l-1 达到了最高的GFOR / GL活性(13.2–13.5 U g-1 干细胞),与用5 g YE l–1 获得的效果。结果证实,CSL是运动发酵单胞菌培养基的一种有效且便宜的补充剂,增加了未经处理的运动发酵单胞菌细胞大规模生物生产山梨糖醇和葡萄糖酸的经济潜力。讨论了该方法的经济可行性。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2001年第4期|442-445|共4页
  • 作者单位

    Instituto de Biotecnologia Universidade de Caxias do Sul C.P. 1352 95001-970 Caxias do Sul Rio Grande do Sul Brazil;

    Universidade Federal de Santa Catarina Florianópolis Santa Catarina Brazil;

    Universidade da Região de Joinville Joinville Santa Catarina Brazil;

    Gesellschaft für Biotechnologische Forschung Mascheroder Weg 1 38124 Braunschweig Germany;

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