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首页> 外文期刊>Applied Microbiology and Biotechnology >Production of glucose-fructose oxidoreductase and ethanol by Zymomonas mobilis ATCC 29191 in medium containing corn steep liquor as a source of vitamins
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Production of glucose-fructose oxidoreductase and ethanol by Zymomonas mobilis ATCC 29191 in medium containing corn steep liquor as a source of vitamins

机译：运动发酵单胞菌ATCC 29191在含有玉米浆作为维生素来源的培养基中生产葡萄糖-果糖氧化还原酶和乙醇

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Different concentrations of corn steep liquor (CSL) were tested in the cultivation of Zymomonas mobilis, Cell growth, ethanol production, and the formation of glucose-fructose oxidoreductase (GFOR) and glucono-delta -lactonase (GL), the enzymes responsible for the bio-production of gluconic acid and sorbitol, were examined. The cell yields using 25 g CSL l(-1) and 40 g CSL l(-1) (Y(X/S)approximate to0.031 g g(-1)) were close to that obtained with 5 g yeast extract (YE) l(-1). With 5 g CSL l(-1) and 15 g CSL l(-1), the nutritional limitation led to smaller Y-X/S Using 100 g CSL l(-1) produced an inhibitory effect on cell growth. Similar ethanol yields (92-95%) were calculated for each concentration of CSL and also for YE medium. The highest specific GFOR/GL activities (13.2-13.5 U g-l dry cell) were reached with 25 g CSL l(-1) and 40 g CSL l(-1), values comparable to that achieved with 5 g YE l(-1). The results confirm that CSL is an effective and cheap supplement fur Z. mobilis medium, increasing the economic potential of a large-scale bio-production of sorbitol and gluconic acid by untreated Z. mobilis cells. The economic feasibility of the process is discussed. [References: 7]
机译：在运动发酵单胞菌的培养，细胞生长，乙醇生产以及葡萄糖-果糖氧化还原酶（GFOR）和葡萄糖酸-δ-内酰胺酶（GL）的形成中测试了不同浓度的玉米浸渍液（CSL）。检查了葡萄糖酸和山梨糖醇的生物生产。使用25 g CSL l（-1）和40 g CSL l（-1）（Y（X / S）大约为0.031 gg（-1））的细胞产量接近5 g酵母提取物（YE ）l（-1）。对于5 g CSL l（-1）和15 g CSL l（-1），营养限制导致较小的Y-X / S。使用100 g CSL l（-1）对细胞生长产生抑制作用。对于每种浓度的CSL以及YE培养基，都计算出了相似的乙醇收率（92-95％）。 25 g CSL l（-1）和40 g CSL l（-1）达到最高的GFOR / GL活性（13.2-13.5 U gl干细胞），其值与5 g YE l（-1）相当）。结果证实，CSL是运动发酵单胞菌的有效且廉价的补充培养基，增加了未经处理的运动发酵单胞菌细胞大规模生物生产山梨糖醇和葡萄糖酸的经济潜力。讨论了该方法的经济可行性。 [参考：7]

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