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首页> 外文期刊>Applied Surface Science >Characterization of bioactive RGD peptide immobilized onto poly(acrylic acid) thin films by plasma polymerization
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Characterization of bioactive RGD peptide immobilized onto poly(acrylic acid) thin films by plasma polymerization

机译：等离子体聚合固定在聚丙烯酸薄膜上的生物活性RGD肽的表征

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摘要

Plasma surface modification can be used to improve the surface properties of commercial pure Ti by creating functional groups to produce bioactive materials with different surface topography. In this study, a titanium surface was modified with acrylic acid (AA) using a plasma treatment and immobilized with bioactive arginine-glycine-aspartic acid (RGD) peptide, which may accelerate the tissue integration of bone implants. Both terminals containing the -NH_2 of RGD peptide sequence and -COOH of poly(acrylic acid) (PAA) thin film were combined with a covalent bond in the presence of l-ethyl-3-3-dimethylaminopropyl carbodiimide (EDC). The chemical structure and morphology of AA film and RGD immobilized surface were investigated by X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FT-IR), atomic force microscopy (AFM), and scanning electron microscopy (SEM). All chemical analysis showed full coverage of the Ti substrate with the PAA thin film containing COOH groups and the RGD peptide. The MC3T3-E1 cells were cultured on each specimen, and the cell alkaline phosphatase (ALP) activity were examined. The surface-immobilized RGD peptide has a significantly increased the ALP activity of MC3T3-E1 cells. These results suggest that the RGD peptide immobilization on the titanium surface has an effect on osteoblastic differentiation of MC3T3-E1 cells and potential use in osteo-conductive bone implants.
机译：等离子体表面改性可用于通过创建官能团来生产具有不同表面形貌的生物活性材料，从而改善商业纯钛的表面性能。在这项研究中，钛的表面经过等离子体处理后被丙烯酸（AA）修饰，并被生物活性精氨酸-甘氨酸-天冬氨酸（RGD）肽固定，这可能会加速骨植入物的组织整合。在1-乙基-3-3-二甲基氨基丙基碳二亚胺（EDC）的存在下，将包含RGD肽序列的-NH_2和-（丙烯酸）（PAA）薄膜的-COOH的两个末端与共价键结合。通过X射线光电子能谱（XPS），傅立叶变换红外光谱（FT-IR），原子力显微镜（AFM）和扫描电子显微镜（SEM）研究了AA薄膜和RGD固定化表面的化学结构和形貌。所有化学分析均显示，含COOH基团和RGD肽的PAA薄膜完全覆盖了Ti基材。在每个标本上培养MC3T3-E1细胞，并检查细胞碱性磷酸酶（ALP）的活性。表面固定的RGD肽显着提高了MC3T3-E1细胞的ALP活性。这些结果表明，RGD肽固定在钛表面上会对MC3T3-E1细胞的成骨细胞分化产生影响，并可能在骨传导性骨植入物中使用。

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  • 来源
    《Applied Surface Science》 |2010年第2期|p.596-602|共7页
  • 作者

    Hyun Suk Seo; Yeong Mu Ko; Jae Won Shim; Yun Kyong Lim; Joong-Ki Kook; Dong-Lyun Cho; Byung Hoon Kim;

  • 作者单位

    Department of Dental Materials, School of Dentistry, MRC Center, Chosun University, Gwangju, South Korea;

    Department of Dental Materials, School of Dentistry, MRC Center, Chosun University, Gwangju, South Korea;

    Department of Dental Materials, School of Dentistry, MRC Center, Chosun University, Gwangju, South Korea;

    Department of Oral Biochemistry, School of Dentistry, Chosun University, Cwangju, South Korea;

    Department of Oral Biochemistry, School of Dentistry, Chosun University, Cwangju, South Korea;

    School of Applied Chemical Engineering and Center for Functional Nano Fine Chemicals, Chonnam National University, Gwangju, South Korea;

    Department of Dental Materials, School of Dentistry,Chosun University, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, South Korea;

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  • 正文语种 eng
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  • 关键词

    acrylic acid (AA); surface modification; plasma polymerization; RGD peptide;

    机译：丙烯酸（AA）;表面改性;等离子体聚合;RGD肽;

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