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A strategy for immunoassay signal amplification using clusters of immunogold nanoparticles

机译：使用免疫金纳米颗粒簇进行免疫分析信号放大的策略

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摘要

The clusters of immunogold nanoparticles were fabricated through cross-linking immunogold nanoparticles with glutaraldehyde. A novel strategy for immunoassay signal amplification using the clusters of immunogold nanoparticles on glass slides based on electroless deposition was described. The immunoassay signal amplification through the clusters of immunogold nanoparticles was about three to four times higher than that through single immunogold nanoparticles. The purple attachments were readily discernible on glass slides by naked eye at a concentration of 0.1 pg/mL antigen in PBS solution. The indirect detection of antigen using the clusters of immunogold nanoparticles is an efficient way to improve the detection sensitivity. (c) 2006 Elsevier B.V. All rights reserved.
机译：通过将免疫金纳米颗粒与戊二醛交联来制备免疫金纳米颗粒的簇。描述了一种新的免疫分析信号放大策略，该方法使用了基于化学沉积的载玻片上的免疫金纳米颗粒簇。通过免疫金纳米颗粒簇的免疫测定信号扩增比通过单个免疫金纳米颗粒的免疫测定信号扩增高约三到四倍。在载玻片上，用PBS溶液中浓度为0.1 pg / mL抗原的肉眼可以很容易地看到紫色的附着物。使用免疫金纳米颗粒簇间接检测抗原是提高检测灵敏度的有效方法。 （c）2006 Elsevier B.V.保留所有权利。

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