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首页> 外文期刊>Archives of Toxicology >Expression of Neuropathy Target Esterase in mouse embryonic stem cells during differentiation
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Expression of Neuropathy Target Esterase in mouse embryonic stem cells during differentiation

机译：神经病性目标酯酶在小鼠胚胎干细胞分化过程中的表达

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Neuropathy Target Esterase (NTE) was initially identified as the primary target esterase of some organophosphorus compounds that cause delayed neuropathy. Some studies in vivo suggest that this protein may also perform a function in embryonic development and therefore also in cell differentiation. The aim of this work was to characterize embryonic stem cells (ESC) as cellular model before to approach to the role of NTE in embryotoxicity processes through mechanistic studies. Mouse D3 ESC in monolayer expressed an NTE activity of 23 nmol phenol/min/mg of protein, while mouse R1 ESC showed a specific NTE activity 3 times higher than D3. An increased expression of gene Pnpla6 (that codifies for NTE) was seen during differentiation in both the D3 cells in monolayer and embryonic bodies (EBS). The maximums of the Pnpla6 expression were reached after 30 h and 5 days of differentiation in monolayer and EBS cultures, respectively. This peak of the Pnpla6 expression correlated with the peak of the NTE enzymatic activity in D3 monolayers. NTE activity and Pnpla6 expression returned to basal levels after 48 h (in monolayer cultures) and 10 days (in EBS) of differentiation, respectively. The changes in the Pnpla6 expression did not correlate with changes noted in the expression of two endoderm, two ectoderm and one neuroectoderm gene markers. In conclusion, this manuscript reports about NTE expression in ESC and its variation during first stages of differentiation. Nevertheless, the role of this activity and the meaning of the variations detected during differentiation must be further studied.
机译：神经病靶标酯酶（NTE）最初被确定为引起延迟神经病的某些有机磷化合物的主要靶标酯酶。体内的一些研究表明，该蛋白还可能在胚胎发育中起作用，因此也可以在细胞分化中起作用。这项工作的目的是在通过机制研究探讨NTE在胚胎毒性过程中的作用之前，将胚胎干细胞（ESC）表征为细胞模型。单层小鼠D3 ESC的NTE活性为23 nmol酚/ min / mg蛋白，而小鼠R1 ESC的特异性NTE活性是D3的3倍。在单层和胚胎小体（EBS）的D3细胞分化过程中，都发现了基因Pnpla6（编码NTE）的表达增加。在单层培养和EBS培养中分别分化30 h和5天后，达到了Pnpla6表达的最大值。 Pnpla6表达的此峰与D3单层中NTE酶活性的峰相关。 NTE活性和Pnpla6表达分别在分化后48小时（在单层培养中）和10天（在EBS中）恢复到基础水平。 Pnpla6表达的变化与两个内胚层，两个外胚层和一个神经外胚层基因标志物的表达中发现的变化不相关。总之，该手稿报道了NTE在ESC中的表达及其在分化初期的变化。然而，这种活动的作用以及分化过程中检测到的变异的含义必须进一步研究。

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