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Molecular Cloning and Evolutionary Analysis of Hemoglobin α-Chain Genes in Bats

机译:蝙蝠血红蛋白α链基因的分子克隆与进化分析

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摘要

Bats are the only mammals with the capacity for powered flight. When flying, they need abundant energy and oxygen. According to previous works, the hemoglobin (Hb) oxygen loading function of bats is insensitive to variations in body temperature, although different bat species have different heat sensitivity. We cloned Hb α-chain sequences from eight bat species to investigate whether they have different characteristics. We found that Hb in the bat lineages is under purifying selection, which accords with the importance of its function in bats. Three turn regions in bat Hb, however, have distinct evolutionary rates compared with those of other mammals, and the codons in these regions have an accelerated rate of evolution. These codons are under divergent selection in bats. These changes in Hb may have occurred in response to the physiological requirements of the species concerned, as adaptations to different lifestyles. Keywords Hemoglobin - α-chain - Gene - Bats - Evolution Yang Liu and Dong Dong contributed equally to this work.
机译:蝙蝠是唯一具有动力飞行能力的哺乳动物。飞行时,它们需要大量的能量和氧气。根据以前的工作,蝙蝠的血红蛋白(Hb)氧负荷功能对体温的变化不敏感,尽管不同的蝙蝠种类对热的敏感性也不同。我们从八个蝙蝠物种中克隆了Hbα-链序列,以研究它们是否具有不同的特征。我们发现蝙蝠血统中的Hb正在纯化选择中,这与其在蝙蝠中功能的重要性相符。然而,与其他哺乳动物相比,蝙蝠Hb中的三个回合区域具有不同的进化速率,并且这些区域中的密码子具有加快的进化速率。这些密码子在蝙蝠中的选择不同。 Hb的这些变化可能是由于适应不同生活方式而对有关物种的生理要求而发生的。关键词血红蛋白-α链-基因-蝙蝠-进化杨柳和董东对这项工作做出了同等贡献。

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