Residue-Specific Description of Non-Native Transient Structures in the Ensemble of Acid-Denatured Structures of the All-β Protein c-src SH3

Heike I. Rsner and Flemming M. Poulsen

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Residue-Specific Description of Non-Native Transient Structures in the Ensemble of Acid-Denatured Structures of the All-β Protein c-src SH3

机译：All-β蛋白c-src SH3的酸变性结构整体中非天然瞬态结构的残基特定描述

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Secondary chemical shift analysis has been used to characterize the unfolded state of acid-ndenatured c-src SH3. Even though native c-src SH3 adopts an all-β fold, we found evidence of transientnhelicity in regions corresponding to native loops. In particular, residues 40-46, connecting the n-src loop tonthe third β-strand, exhibited an apparent helicity of nearly 45%. Furthermore, the RT loop and the divergingnturn appeared to adopt non-native-like helical conformations. Interestingly, none of the residues found inntransient helical conformations exhibited significant φ-values [Riddle, D. S., et al. (1999) Nat. Struct. Biol. 6,n1016-1024]. This indicated that the transient helicity has no influence or only a weak influence on the actualnprotein folding reaction. The residual structural propensities were compared to those of other SH3 domains,nrevealing heterogeneity in the unfolded ensemble that clearly contrasts with the conserved character of thentopology of native state and transition state ensembles typical for SH3 domains.

机译：二级化学位移分析已用于表征酸未改性的c-src SH3的未折叠状态。即使天然c-src SH3采用全β折叠，我们也发现了与天然环相对应的区域发生瞬变的证据。特别地，连接n-src环与第三条β链的残基40-46表现出近45％的表观螺旋度。此外，RT环和发散角似乎采用了非天然的螺旋构象。有趣的是，在非瞬时螺旋构象中发现的残基均未显示出显着的φ值[Riddle，D.S。，等。（1999）Nat。结构。生物学6，n1016-1024]。这表明瞬时螺旋度对实际的蛋白折叠反应没有影响或只有很小的影响。将其余的结构倾向与其他SH3结构域进行了比较，揭示了未折叠集合中的异质性，这与SH3结构域典型的自然状态和过渡态集合的拓扑学的保守特性形成鲜明对比。

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《Biochemistry》 |2010年第15期|p.3246-3253|共8页
作者
Heike I. Rsner and Flemming M. Poulsen;
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Structure Biology and NMR Laboratory, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5,DK-2200 Copenhagen N, Denmark;

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1. Residue-Specific Description of Non-Native Transient Structures in the Ensemble ofAcid-Denatured Structures of the All-13 Protein c-src SH3t [J] . Heike I. Rosner, Flemming M. Poulsen Biochemistry . 2010,第15期

机译：在全-13蛋白C-SRC SH3T的酸的刚性变性结构中的非天然瞬态结构的特异性描述
2. Improved structural characterizations of the drkN SH3 domain unfolded state suggest a compact ensemble with native-like and non-native structure. [J] . Marsh JA, Neale C, Jack FE, Journal of Molecular Biology . 2007,第5期

机译：drkN SH3结构域的未折叠状态的改进的结构特征表明具有天然样和非天然结构的紧凑的整体。
3. The effect of an engineered ATCUN motif on the structure and biophysical properties of the SH3 domain of c-Src tyrosine kinase [J] . Journal of biological inorganic chemistry: JBIC: a publication of the Society of Biological Inorganic Chemistry . 2020,第4期

机译：工程ATCUN基序对C-SRC酪氨酸激酶SH3结构域的结构和生物物理性质的影响
4. Characterization of Domain-Peptide Interaction Interface: Prediction of SH3 Domain-Mediated Protein-Protein Interaction Network in Yeast by Generic Structure-Based Models [C] . Tingjun Hou, Nan Li, Youyong Li, International conference of molecular simulations and applied informatics technologies . 2012

机译：域-肽相互作用界面的表征：基于通用结构的模型预测酵母中SH3域介导的蛋白质-蛋白质相互作用网络。
5. Three dimensional structures of SH2 and SH3 domains of the Abl and Shc oncoproteins. [D] . O, Hyun-Joo Nam. 1996

机译：Abl和Shc癌蛋白的SH2和SH3域的三维结构。
6. Structures of an all-α protein running along the DNA major groove [O] . Li-Yan Yu, Wang Cheng, Kang Zhou, 2016

机译：沿DNA主沟延伸的全α蛋白质的结构
7. Intertwined dimeric structure for the SH3 domain of the c-Src tyrosine kinase induced by polyethylene glycol binding [O] . Cámara-Artigas Ana, Martín-García José M., Morel Bertrand, 2009

机译：聚乙二醇结合诱导的c-Src酪氨酸激酶SH3结构域的二元结构交织在一起

Residue-Specific Description of Non-Native Transient Structures in the Ensemble of Acid-Denatured Structures of the All-β Protein c-src SH3

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