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首页> 外文期刊>Biomarkers >The combination of urine DD3~(PCA3) mRNA and PSA mRNA as molecular markers of prostate cancer
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The combination of urine DD3~(PCA3) mRNA and PSA mRNA as molecular markers of prostate cancer

机译：尿DD3〜（PCA3）mRNA和PSAmRNA的组合作为前列腺癌的分子标记

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摘要

The identification of reliable molecular biomarkers in prostate cancer early diagnosis is clinically desirable. We quantitatively detected prostate cancer specifically overexpressed genes, DD3 and PSA, in urine sediments of men suffering from prostate cancer or benign prostate hyperplasia, after prostatic massage. As both genes are exclusively expressed in androgen receptor expressing human prostate carcinoma cell lines, we further investigated the possible effect of androgens on PSA and DD3 gene expression. DD3 and PSA mRNA levels were measured by real-time polymerase chain reaction. The combined markers test had a sensitivity of 80.2% and a specificity of 100%. Both gene transcripts were significantly upregulated by androgens. Results indicated the clinical usefulness of the combination of DD3 and PSA as molecular markers in the early diagnosis of prostate cancer and the need of combining as many as possible analytical data with the clinical and demographic ones to achieve the maximum level of diagnostic accuracy.
机译：在前列腺癌的早期诊断中鉴定可靠的分子生物标志物是临床上需要的。在前列腺按摩后，我们定量检测了患有前列腺癌或良性前列腺增生的男性尿液中的前列腺癌，特别是过量表达的基因DD3和PSA。由于这两个基因仅在表达雄激素受体的人前列腺癌细胞系中表达，因此我们进一步研究了雄激素对PSA和DD3基因表达的可能影响。通过实时聚合酶链反应测量DD3和PSA mRNA水平。组合标记测试的灵敏度为80.2％，特异性为100％。两种基因转录物均被雄激素显着上调。结果表明，DD3和PSA的组合作为分子标记物在前列腺癌的早期诊断中的临床有用性，并且需要将尽可能多的分析数据与临床和人口统计数据相结合，以实现最大的诊断准确性。

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  • 来源
    《Biomarkers》 |2009年第4期|235-243|共9页
  • 作者

    Ettore Mearini; Cinzia Antognelli; Chiara Del Buono; Giovanni Cochetti; Antonella Giannantoni; Emanuela Nardelli; Vincenzo N. Talesa;

  • 作者单位

    Department of Medical-Surgical Specialties and Public Health, Division of Urology and Andrology, University of Perugia, Perugia, Italy University of Perugia, Department of Medical-Surgical Specialties and Public Health, Division of Urology and Andrology, via Brunamonti 06126, Perugia, Italy;

    University of Perugia, Department of Experimental Medicine, via del Giochetto 06123, Perugia, Italy;

    University of Perugia, Department of Experimental Medicine, via del Giochetto 06123, Perugia, Italy;

    University of Perugia, Department of Medical-Surgical Specialties and Public Health, Division of Urology and Andrology, via Brunamonti 06126, Perugia, Italy;

    University of Perugia, Department of Medical-Surgical Specialties and Public Health, Division of Urology and Andrology, via Brunamonti 06126, Perugia, Italy;

    University of Perugia, Department of Experimental Medicine, via del Giochetto 06123, Perugia, Italy;

    University of Perugia, Department of Experimental Medicine, via del Giochetto 06123, Perugia, Italy;

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  • 关键词

    prostate cancer; DD3 mRNA; PSA mRNA; molecular markers; benign prostatic hyperplasia; urine sediments;

    机译：前列腺癌;DD3 mRNA;PSA mRNA;分子标记良性前列腺增生症;尿沉渣;

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