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Development of a cost-effective production process for Halomonas levan

机译：开发高成本的Halomonas levan生产工艺

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摘要

Levan polysaccharide is an industrially important natural polymer with unique properties and diverse high-value applications. However, current bottlenecks associated with its large-scale production need to be overcome by innovative approaches leading to economically viable processes. Besides many mesophilic levan producers, halophilic Halomonas smyrnensis cultures hold distinctive industrial potential and, for the first time with this study, the advantage of halophilicity is used and conditions for non-sterile levan production were optimized. Levan productivity of Halomonas cultures in medium containing industrial sucrose from sugar beet and food industry by-product syrup, a total of ten sea, lake and rock salt samples from four natural salterns, as well as three different industrial-grade boron compounds were compared and the most suitable low-cost substitutes for sucrose, salt and boron were specified. Then, the effects of pH control, non-sterile conditions and different bioreactor modes (batch and fed-batch) were investigated. The development of a cost-effective production process was achieved with the highest yield (18.06 g/L) reported so far on this microbial system, as well as the highest theoretical bioconversion efficiency ever reported for levan-producing suspension cultures. Structural integrity and biocompatibility of the final product were also verified in vitro.
机译：莱万多糖是工业上重要的天然聚合物，具有独特的性能和多种高价值的应用。但是，需要通过创新的方法来克服当前与其大规模生产相关的瓶颈，以实现经济可行的流程。除了许多中温莱文生产者以外，嗜盐嗜盐单胞菌培养物具有独特的工业潜力，并且本研究首次利用了嗜盐性的优势，并优化了非无菌莱文生产的条件。比较了含有甜菜和食品工业副糖浆工业蔗糖的培养基中Halomonas培养基的Levan生产力，来自四个天然盐厂的总共十个海盐，湖泊盐和岩盐样品以及三种不同的工业级硼化合物的比重。确定了最合适的蔗糖，盐和硼的低成本替代品。然后，研究了pH控制，非无菌条件和不同生物反应器模式（分批和补料分批）的影响。开发了具有成本效益的生产工艺，迄今为止，该微生物系统报告的产量最高（18.06 g / L），而生产Levan的悬浮培养物的理论生物转化效率也最高。最终产品的结构完整性和生物相容性也在体外进行了验证。

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