• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >Effects of betaine supplementation on L-threonine fed-batch fermentation by Escherichia coli
【24h】

Effects of betaine supplementation on L-threonine fed-batch fermentation by Escherichia coli

机译：添加甜菜碱对大肠杆菌L-苏氨酸分批补料发酵的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Betaine can act as a stress protectant, methyl donor, or enzyme stabilizer in vitro for the biosynthesis of structurally complex compounds. The performances of betaine type and concentration on the metabolic processes of Escherichia coli JLTHR in a 5-L fermentor were investigated. The results showed that the maximum L-threonine production of 127.3 g/L and glucose conversion percentage of 58.12% was obtained fed with the glucose solution containing 2 g/L betaine hydrochloride, which increased by 14.5 and 6.87% more compared to that of the control, respectively. This study presents an analysis of the metabolic fluxes of E. coli JLTHR for the production of L-threonine with betaine supplementation. When betaine was fed into the fermentation culture medium, the metabolic flux entering into the pentose phosphate pathway (HMP) and biosynthesis route of L-threonine increased by 57.3 and 10.1%, respectively. In conclusion, exogenous addition of betaine was validated to be a feasible and efficacious approach to improve L-threonine production.
机译：甜菜碱可以在体外用作生物保护结构复杂化合物的应力保护剂，甲基供体或酶稳定剂。研究了甜菜碱类型和浓度对5-L发酵罐中大肠杆菌JLTHR代谢过程的影响。结果表明，加入含2 g / L甜菜碱盐酸盐的葡萄糖溶液，可得到的最大L-苏氨酸产量为127.3 g / L，葡萄糖转化率为58.12％，比甜菜碱盐酸盐分别增加14.5和6.87％。控制。这项研究对补充甜菜碱的L-苏氨酸生产大肠杆菌JLTHR的代谢通量进行了分析。将甜菜碱加入发酵培养基后，进入戊糖磷酸途径（HMP）和L-苏氨酸生物合成途径的代谢通量分别增加了57.3％和10.1％。总之，外源添加甜菜碱被证实是提高L-苏氨酸产量的可行且有效的方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号