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Efficient utilization of Eucheuma denticulatum hydrolysates using an activated carbon adsorption process for ethanol production in a 5-L fermentor

机译:使用活性炭吸附工艺有效利用细叶细叶果水解产物在5-L发酵罐中生产乙醇

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摘要

A total monosaccharide concentration of 37.8 g/L and 85.9% conversion from total fermentable monosaccharides of 44.0 g/L from 110 g dw/L Eucheuma denticulatum slurry were obtained by thermal acid hydrolysis and enzymatic saccharification. Subsequent adsorption treatment to remove 5-hydroxymethylfurfural (5-HMF) using 5% activated carbon and an adsorption time of 10 min were used to prevent a prolonged lag phase, reduced cell growth, and low ethanol production. The equilibrium adsorption capacity (q (e)) of HMF (58.183 mg/g) showed high affinity to activated carbon comparing to those of galactose (2.466 mg/g) and glucose (2.474 mg/g). The efficiency of cell growth and ethanol production with activated carbon treatment was higher than that without activated carbon treatment. Fermentation using S. stipitis KCTC7228 produced a cell concentration of 3.58 g dw/L with Y (X/S) of 0.107, and an ethanol concentration of 15.8 g/L with Y (P/S) of 0.48 in 96 h.
机译:通过热酸水解和酶促糖化,从110 g dw / L的Eucheuma denticulatum浆液中得到的总单糖浓度为37.8 g / L,由总可发酵单糖的44.0 g / L转化率为85.9%。随后的吸附处理使用了5%的活性炭去除5-羟甲基糠醛(5-HMF),吸附时间为10分钟,以防止延长的滞后阶段,减少的细胞生长和低乙醇生产。与半乳糖(2.466 mg / g)和葡萄糖(2.474 mg / g)相比,HMF(58.183 mg / g)的平衡吸附容量(q(e))对活性炭具有较高的亲和力。用活性炭处理的细胞生长和乙醇生产的效率要高于不使用活性炭处理的细胞。使用S.stipitis KCTC7228进行发酵在96小时内产生的细胞浓度为3.58 g dw / L,Y(X / S)为0.107,乙醇浓度为15.8 g / L,Y(P / S)为0.48。

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