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首页> 外文期刊>Phytochemistry >HALOCARBON PRODUCTION AND IN VIVO BROMINATING ACTIVITY OF EUCHEUMA DENTICULATUM
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HALOCARBON PRODUCTION AND IN VIVO BROMINATING ACTIVITY OF EUCHEUMA DENTICULATUM

机译：CHE草的碳生产和体内溴化活性

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摘要

In vivo brominating activity by Eucheuma denticulatum was compared with its production of volatile halocarbons. The ability of the alga to brominate phenol red showed good correlation (p < 0.05, r = 1.0) to the production of bromoform (CHBr3) estimated by gas chromatography. The production of tetrabromophenol or bromoform was raised by exposing the algae to mechanical stress (in the form of cutting), increased light or addition of the herbicide DCMU, prior to the measurements. We suggest that the in vivo brominating activity of E. denticulatum can be used as a simple method to study the physiology of this alga, We also suggest that the activity of the brominating peroxidases is dependent on the intracellular concentration of H2O2, possibly produced by the Mehler reaction. Production of singlet oxygen and triplet chlorophyll by photo-oxidative damage may increase halocarbon release. The amount produced and the species of volatile halocarbons released are dependent on the conditions the algae are subjected to prior and during the experiment. Addition of NaCN or NaN3 inhibited the brominating activity with 95 or 75% of control values.
机译：将Eucheuma denticulatum的体内溴化活性与其挥发性卤代烃的产生进行了比较。藻类溴化酚红的能力与气相色谱法估计的溴代甲烷（CHBr3）产生了很好的相关性（p <0.05，r = 1.0）。在测量之前，通过将藻类暴露于机械应力（以切割的形式），增加光照或添加除草剂DCMU，可以提高四溴酚或溴仿的产量。我们建议，可以将E. denticulatum的体内溴化活性用作研究该藻类生理的简单方法。我们还建议溴化过氧化物酶的活性取决于细胞内H2O2的浓度，该浓度可能是由H2O2产生的。梅勒反应。光氧化损伤产生单线态氧和三线态叶绿素可能会增加卤代烃的释放。产生的量和释放的挥发性卤代烃的种类取决于藻类在实验之前和实验过程中所处的条件。 NaCN或NaN3的添加抑制溴化活性，达到控制值的95％或75％。

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