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首页> 外文期刊>Bioprocess and Biosystems Engineering >Effect of pH on the metabolic flux of Klebsiella oxytoca producing 2,3-butanediol in continuous cultures at different dilution rates
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Effect of pH on the metabolic flux of Klebsiella oxytoca producing 2,3-butanediol in continuous cultures at different dilution rates

机译：pH值对不同稀释度连续培养中产酸克雷伯菌2,3-丁二醇代谢通量的影响

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摘要

The efficiency of the bioconversion process and the achievable end-product concentration decides the economic feasibility of microbial 2,3-butanediol (2,3-BDO) production. In 2,3-BDO production, optimization of culture condition is required for cell growth and metabolism. Also, the pH is an important factor that influences microbial performance. For different microorganisms and substrates, it has been shown that the distribution of the metabolites in 2,3-BDO fermentation is greatly affected by pH, and the optimum pH for 2,3-BDO production seems dependently linked to the particular strain and the substrate employed. Quantification analysis of intracellular metabolites and metabolic flux analysis (MFA) were used to investigate the effect of pH on the Klebsiella oxytoca producing 2,3-BDO and other organic acids. The main objectives of MFA are the estimation of intracellular metabolic fluxes and the identification of rate-limiting step and the key enzymes. This study was conducted under continuous aerobic conditions at different dilution rates (0.1,0.2, and 0.3 h~(-1)) and different pH values (pH 5.5 and 7.0) for the steady-state experimental data. In order to obtain the flux distribution, the extracellular specific rates were calculated from the experimental data using the metabolic network model of K. oxytoca. Intracellular metabolite concentration profiles were generated using ultra-performance liquid chro-matography/quadrupole time-of-flight mass spectrometry.
机译：生物转化过程的效率和可达到的终产物浓度决定了微生物2,3-丁二醇（2,3-BDO）生产的经济可行性。在2,3-BDO生产中，需要优化培养条件以促进细胞生长和代谢。而且，pH是影响微生物性能的重要因素。对于不同的微生物和底物，已表明2,3-BDO发酵中代谢物的分布受pH的影响很大，而2,​​3-BDO生产的最佳pH似乎与特定菌株和底物有关。受雇。细胞内代谢产物的定量分析和代谢通量分析（MFA）用于研究pH对产2,3-BDO和其他有机酸的产酸克雷伯菌的影响。 MFA的主要目标是估计细胞内代谢通量以及鉴定限速步骤和关键酶。该研究是在连续有氧条件下，以不同的稀释率（0.1、0.2和0.3 h〜（-1））和不同的pH值（pH 5.5和7.0）进行的，用于稳态实验数据。为了获得通量分布，使用催产克雷伯氏菌的代谢网络模型从实验数据计算细胞外比率。使用超高效液相色谱/四极杆飞行时间质谱仪生成细胞内代谢物浓度曲线。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2013年第6期|845-855|共11页
  • 作者

    Changhun Park; Mingshou Lu; Seokhun Yun; Kyungmoon Park; Jinwon Lee;

  • 作者单位

    Department of Chemical and Biomolecular Engineering,Sogang University, Seoul 121-742, Republic of Korea;

    Department of Chemical and Biomolecular Engineering,Sogang University, Seoul 121-742, Republic of Korea;

    Department of Chemical and Biomolecular Engineering,Sogang University, Seoul 121-742, Republic of Korea,Research Institute of Biotechnology, CJ CheilJedang, Seoul 157-724. Republic of Korea;

    Department of Biological and Chemical Engineering,Hongik University, Chungcheongnam-do 339-701,Republic of Korea;

    Department of Chemical and Biomolecular Engineering,Sogang University, Seoul 121-742, Republic of Korea;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Metabolic flux analysis; Klebsiella oxytoca; 2,3-Butanediol; Continuous cultures; pH;

    机译：代谢通量分析;产酸克雷伯菌2,3-丁二醇;连续文化;pH值;

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