...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >Application of solid-phase extraction to agar-supported fermentation
【24h】

Application of solid-phase extraction to agar-supported fermentation

机译：固相萃取在琼脂辅助发酵中的应用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Agar-supported fermentation (Ag-SF), a variant of solid-state fermentation, has recently been improved by the development of a dedicated 2 m~2 scale pilot facility, Platotex. We investigated the application of solid-phase extraction (SPE) to Ag-SF in order to increase yields and minimize the contamination of the extracts with agar constituents. The selection of the appropriate resin was conducted on liquid-state fermentation and Diaion HP-20 exhibited the highest recovery yield and selectivity for the metabolites of the model fungal strains Phomopsis sp. and Fusarium sp. SPE applied to Ag-SF resulted in a particular compartmentalization of the culture. The mycelium that requires oxygen to grow migrates to the top layer and formed a thick biofilm. The resin beads intercalate between the agar surface and the mycelium layer, and trap directly the compounds secreted by the mycelium through a "solid-solid extraction" (SSE) process. The resin/mycelium layer is easily recovered by scraping the surface and the target metabolites extracted by methanol. Ag-SF associated to SSE represents an ideal compromise for the production of bioactive secondary metabolites with limited economic and environmental impact.
机译：琼脂支持的发酵（Ag-SF）是固态发酵的一种变体，最近通过开发专用的2 m〜2规模的中试设备Platotex进行了改进。我们研究了将固相萃取（SPE）应用于Ag-SF的方法，以提高产量并最大程度地减少萃取液对琼脂成分的污染。在液态发酵中选择合适的树脂，Diaion HP-20对模型真菌菌株Phomopsis sp。的代谢产物具有最高的回收率和选择性。和镰刀菌应用于Ag-SF的SPE导致培养物的特定分隔。需要氧气生长的菌丝体迁移到顶层并形成厚的生物膜。树脂珠插入琼脂表面和菌丝体层之间，并通过“固-固萃取”（SSE）过程直接捕获菌丝体分泌的化合物。通过刮擦表面和甲醇提取的目标代谢物，可以轻松回收树脂/菌丝体层。与SSE相关的Ag-SF代表了对生产具有经济和环境影响有限的生物活性次生代谢产物的理想折衷方案。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2013年第9期|1285-1290|共6页
  • 作者

    Geraldine Le Goff; Emilie Adelin; Sylvie Cortial; Claudine Servy; Jamal Ouazzani;

  • 作者单位

    Centre de Recherche de Git, lnstitut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (C.N.R.S), Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex,France;

    Centre de Recherche de Git, lnstitut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (C.N.R.S), Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex,France;

    Centre de Recherche de Git, lnstitut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (C.N.R.S), Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex,France;

    Centre de Recherche de Git, lnstitut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (C.N.R.S), Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex,France;

    Centre de Recherche de Git, lnstitut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (C.N.R.S), Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex,France;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Solid-state fermentation; Agar-supported fermentation; Solid-phase extraction; Solid-solid extraction; Microbial secondary metabolites; Neutral resins;

    机译：固态发酵;琼脂支持的发酵;固相萃取;固-固萃取;微生物次生代谢产物;中性树脂;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号