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首页> 外文期刊>Biotechnology and bioprocess engineering >Optimization Of Culture Conditions For1,3-propanediol Production From Crude glycerol By Klebsiella Pneumoniae Using response Surface Methodology
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Optimization Of Culture Conditions For1,3-propanediol Production From Crude glycerol By Klebsiella Pneumoniae Using response Surface Methodology

机译:响应面法优化肺炎克雷伯菌从粗甘油生产1,3-丙二醇的培养条件

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摘要

To produce 1,3-propanediol (1,3-PD) from crude glycerol, cultivation conditions were optimized by response surface methodology (RSM) based on a 2~5 factorial central composite design (CCD). RSM was adopted to derive a statistical model for the individual and interactive effects of crude glycerol, (NH_4)_2SO_4, pH, cultivation time and temperature on the production of 1,3-PD. Optimal conditions for maximum 1,3-PD production were as follows: crude glycerol, 35 g/L; (NH_4)_2SO_4, 8 g/L; pH, 7.37; cultivation time, 10.8 h; temperature, 36.88℃. Under these optimal conditions, the design expert presented the maximal numerical solution with a predicted 1,3-PD production level of up to 13.74 g/L. The experimental production of 1,3-PD yielded 13.8 g/L, which was in close agreement with the model prediction.
机译:为了从粗甘油生产1,3-丙二醇(1,3-PD),通过基于2〜5阶乘中心复合设计(CCD)的响应面法(RSM)优化了培养条件。采用RSM推导了粗甘油,(NH_4)_2SO_4,pH,培养时间和温度对1,3-PD生产的个体和相互作用影响的统计模型。最大化1,3-PD生产的最佳条件如下:粗甘油35 g / L; (NH_4)_2SO_4,8 g / L; pH值7.37;培养时间10.8小时;温度36.88℃。在这些最佳条件下,设计专家提出了最大的数值解决方案,其预测的1,3-PD生产水平高达13.74 g / L。 1,3-PD的实验产量为13.8 g / L,与模型预测非常吻合。

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